Expression of VOPP1 in hepatocellular carcinoma and its relationship with prognosis

doi:10.3877/cma.j.issn.2095-3232.2024026

Abstract

Abstract:

Objective

To investigate the expression of vesicular over-expressed in cancer prosurvival protein 1 （VOPP1） in hepatocellular carcinoma （HCC） and its relationship with clinical prognosis of HCC patients.

Methods

Clinicopathological data of 90 HCC patients who underwent radical resection in the Third Affiliated Hospital of Sun Yat-sen University from February 2008 to August 2015 were retrospectively analyzed. The informed consents of all patients were obtained and the local ethical committee approval was received. Among them，81 patients were male and 9 female，aged 27-74 years，with a median age of 53 years. 11 cases were classified as TNM stage Ⅰ，29 cases of TNM stage Ⅱ，26 cases of TNM stage Ⅲ and 2 cases of TNM stage Ⅳ. The expression level of VOPP1 in HCC and adjacent tissues was determined by immunohistochemical staining，and the relationship between VOPP1 expression and clinicopathological parameters was analyzed. In addition，the expression level of VOPP1 mRNA in the cancer and adjacent tissues of 10 HCC patients was detected by qRT-PCR. Survival analysis was conducted by Kaplan-Meier method and Log-rank test. The independent risk factors of clinical prognosis were assessed by Cox regression model.

Results

qRT-PCR showed that the average relative expression of VOPP1 mRNA in HCC tissues was （4.91±1.98）×10^-3，significantly higher than （0.39±0.12）×10^-3 in paired adjacent tissues （t=2.284， P＜0.05）. Immunohistochemical staining showed that the positive expression rate of VOPP1 in HCC was 52%（47/90），significantly higher than 28%（25/90） in adjacent tissues （χ²=11.204， P＜0.05）.The high expression of VOPP1 in HCC tissues was correlated with histological grade （χ²=9.373， P＜0.05）.Survival analysis showed that the median recurrence-free survival （RFS） of HCC patients with high and low expression of VOPP1 after radical resection was 17 and 50 months，respectively. The RFS of patients with high expression of VOPP1 was significantly lower than that of their counterparts with low expression of VOPP1 （χ²=7.769， P＜0.05）. Cox regression analysis showed that T3 stage （HR=3.649，95%CI: 1.278-10.420），T4 stage （HR=6.157，95%CI: 1.297-29.235） and high expression of VOPP1 protein （HR=1.866，95%CI: 1.069-3.258） were the independent risk factors of postoperative RFS （P＜0.05）.

Conclusions

VOPP1 is highly expressed in HCC tissues. The expression level of VOPP1 is negatively correlated with clinical prognosis. VOPP1 is an independent risk factor of clinical prognosis of HCC，which can be utilized as a new and valuable prognostic factor.

Key words: Carcinoma，hepatocellular, Prognosis, VOPP1, Tumor markers

Xiaopeng Chen, Jiani Wang, Qinghai Lian, Jiumei Yang. Expression of VOPP1 in hepatocellular carcinoma and its relationship with prognosis[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2024, 13(06): 876-882.

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Figures/Tables 5

References 23

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指标	例数	VOPP1低表达组	VOPP1高表达组	χ ²值	P值
男性	81	40	41	0.836	0.360
年龄≥60岁	24	10	14	0.490	0.484
HBV感染	85	39	46	2.203	0.138
肿瘤直径≥5cm	39	16	23	1.258	0.262
组织学分级				9.373	0.007
Ⅰ级	3	2	1
Ⅱ级	54	26	28
Ⅲ级	33	15	18
AJCC分期				-	0.463
Ⅰ期	11	7	4
Ⅱ期	29	14	15
Ⅲ期	48	22	26
Ⅳ期	2	0	2

指标	例数	VOPP1低表达组	VOPP1高表达组	χ ²值	P值
男性	81	40	41	0.836	0.360
年龄≥60岁	24	10	14	0.490	0.484
HBV感染	85	39	46	2.203	0.138
肿瘤直径≥5cm	39	16	23	1.258	0.262
组织学分级				9.373	0.007
Ⅰ级	3	2	1
Ⅱ级	54	26	28
Ⅲ级	33	15	18
AJCC分期				-	0.463
Ⅰ期	11	7	4
Ⅱ期	29	14	15
Ⅲ期	48	22	26
Ⅳ期	2	0	2

参数	单因素分析			多因素分析
参数	HR	95%CI	P值	HR	95%CI	P值
HBV感染	2.797	1.102～7.096	0.030
肿瘤直径≥5cm	2.065	1.219～3.500	0.007
AJCC分期			0.001
Ⅰ期	参考
Ⅱ期	1.520	0.494～4.673	0.465
Ⅲ期	4.098	1.445～11.617	0.008
Ⅳ期	14.278	2.505～81.377	0.003
VOPP1高表达	2.104	1.225～3.613	0.007	1.866	1.069～3.258	0.028
T分期			0.001			0.003
1期	参考			参考
2期	1.519	0.494～4.672	0.465	1.370	0.444～4.232	0.584
3期	4.075	1.436～11.567	0.008	3.649	1.278～10.420	0.016
4期	8.727	1.892～40.251	0.005	6.157	1.297～29.235	0.022

参数	单因素分析			多因素分析
参数	HR	95%CI	P值	HR	95%CI	P值
HBV感染	2.797	1.102～7.096	0.030
肿瘤直径≥5cm	2.065	1.219～3.500	0.007
AJCC分期			0.001
Ⅰ期	参考
Ⅱ期	1.520	0.494～4.673	0.465
Ⅲ期	4.098	1.445～11.617	0.008
Ⅳ期	14.278	2.505～81.377	0.003
VOPP1高表达	2.104	1.225～3.613	0.007	1.866	1.069～3.258	0.028
T分期			0.001			0.003
1期	参考			参考
2期	1.519	0.494～4.672	0.465	1.370	0.444～4.232	0.584
3期	4.075	1.436～11.567	0.008	3.649	1.278～10.420	0.016
4期	8.727	1.892～40.251	0.005	6.157	1.297～29.235	0.022

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