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中华肝脏外科手术学电子杂志 ›› 2012, Vol. 01 ›› Issue (01) : 46 -50. doi: 10.3877/cma.j.issn.2095-3232.2012.01.011

所属专题: 文献

基础研究

基质金属蛋白酶2和Ⅳ型胶原在肝细胞肝癌组织中的表达及意义
王慧玲1, 区应亮1, 简志祥1,()   
  1. 1. 510080 广州,广东省人民医院(广东省医学科学院)肝胆外科
  • 收稿日期:2012-04-05 出版日期:2012-08-10
  • 通信作者: 简志祥
  • 基金资助:
    广东省自然科学基金(2009B080701021)

Expression and significance of MMP-2 and collagen Ⅳ protein in hepatocellular carcinoma

Hui-ling WANG1, Ying-liang OU1, Zhi-xiang JIAN1,()   

  1. 1. Department of Hepatic Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou 510080, China
  • Received:2012-04-05 Published:2012-08-10
  • Corresponding author: Zhi-xiang JIAN
  • About author:
    Corresponding author: JIAN Zhi-xiang, Email:
引用本文:

王慧玲, 区应亮, 简志祥. 基质金属蛋白酶2和Ⅳ型胶原在肝细胞肝癌组织中的表达及意义[J]. 中华肝脏外科手术学电子杂志, 2012, 01(01): 46-50.

Hui-ling WANG, Ying-liang OU, Zhi-xiang JIAN. Expression and significance of MMP-2 and collagen Ⅳ protein in hepatocellular carcinoma[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2012, 01(01): 46-50.

目的

探讨基质金属蛋白酶2(MMP-2)和Ⅳ型胶原在肝细胞肝癌(肝癌)患者的肝癌组织中表达及意义。

方法

本回顾性研究选择2005年1月至2007年12月广东省人民医院肝胆外科40例肝癌患者的肝切除标本(肝癌组)为研究对象,另取5例尸检来源的正常肝组织作为正常对照组,所有患者均签署知情同意书,符合医学伦理学规定。应用免疫组织化学方法检测其肝组织的MMP-2和Ⅳ型胶原表达情况,用Spearman相关检验分析MMP-2、IV型胶原与肝癌微血管浸润、肿瘤直径、分化程度、肿瘤复发的关系。

结果

MMP-2和Ⅳ型胶原在肝癌组的阳性表达分别为29例(73%)、38例(95%),MMP-2在正常肝组织中无表达,Ⅳ型胶原在正常对照组中阳性表达为3例。MMP-2和IV型胶原在肝癌组织表达率增高。MMP-2和Ⅳ型胶原均与肝癌的复发有关(均为P<0.05),当有微血管浸润时,MMP-2和Ⅳ型胶原的阳性率均显著增高(均为P<0.05)。

结论

MMP-2和Ⅳ型胶原在肝癌组织中呈阳性表达,且对判断肝癌患者术后复发有一定意义。

Objective

To study the expression and significance of matrix metalloproteinase-2 (MMP-2) and collagen Ⅳ protein (collagen Ⅳ) in liver tissues of hepatocellular carcinoma (HCC) .

Methods

Forty HCC tissues were collected from the hepatic resection specieces in department of hepatic surgery in Guangdong General Hospital from Janaury 2005 to December 2007 and stained by immunohistochemistry to observe the expression of MMP-2 and collagen Ⅳ. Five normal liver tissues were obtained by autopsy as control. Local ethical committee approval had been received and that the informed concent of all participating subjects was obtained. The relationship between MMP-2, collagen Ⅳ expression and clinicopathologic features of HCC such as microvascular invasion, tumor diameter, tumor differentiation degree, recurrence was analyzed by test of Spearman’s rank-correlation coefficient.

Results

The positive expression of MMP-2 and collagen Ⅳ in HCC tissues were 29 cases(73%) and 38 cases(95%) respectively. The MMP-2 expression was negative in normal liver tissues, while the expression of collagen Ⅳ was observed in 3 samples among 5 tested normal liver tissues. The expressions of MMP-2 and collagen Ⅳ were related to the recurrence of HCC (all in P<0.05) . The positive expression rate of MMP-2 and collagen Ⅳ in HCC was markedly related to the microvascular invasion (all in P<0.05) .

Conclusions

The expressions of MMP-2 and collagen Ⅳ are positive in HCC tissues and may relate to the recurrence of hepatocellular carcinoma.

图1 MMP-2在正常肝组织中呈阴性表达(SP法,×100)
图2 MMP-2在肝癌组织中呈强阳性表达(SP法,×100)
图3 Ⅳ型胶原在正常肝组织中呈阴性表达(SP法,×100)
图4 Ⅳ型胶原在肝癌组织中呈强阳性表达(SP法,×100)
表1 两组肝组织MMP-2表达的比较(例)
表2 两组肝组织Ⅳ型胶原表达的比较(例)
表3 MMP-2表达与肝癌病理因素及复发的关系(例)
表4 Ⅳ型胶原表达与肝癌病理因素及复发的关系(例)
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