切换至 "中华医学电子期刊资源库"
高级检索
中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2015, Vol. 04 ›› Issue (02) : 125 -128. doi: 10.3877/cma.j.issn.2095-3232.2015.02.015

所属专题： 文献；

基础研究

ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用
邓量1, 梁明懿2, 张昆松3,†(), 许峰峰1, 黎文峰1, 张继红1   
  1. 1. 510080　广州，中山大学附属第一医院东院肝胆外科
    2. 510080　广州，中山大学附属第一医院妇产科
    3. 510080　广州，中山大学附属第一医院胆胰外科
  • 收稿日期:2014-10-19 出版日期:2015-04-10
  • 通信作者: 张昆松
  • 基金资助:
    广东省自然科学基金(S2012040006299，10451008901005954)

Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp

Liang Deng1, Mingyi Liang2, Kunsong Zhang3(), Fengfeng Xu1, Wenfeng Li1, Jihong Zhang1   

  1. 1. Department of Hepatobiliary Surgery, East Branch, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
    2. Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
    3. Department of Biliary Pancreatic Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
  • Received:2014-10-19 Published:2015-04-10
  • Corresponding author: Kunsong Zhang
  • About author:
    Corresponding author: Zhang Kunsong, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

邓量, 梁明懿, 张昆松, 许峰峰, 黎文峰, 张继红. ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用[J]. 中华肝脏外科手术学电子杂志, 2015, 04(02): 125-128.

Liang Deng, Mingyi Liang, Kunsong Zhang, Fengfeng Xu, Wenfeng Li, Jihong Zhang. Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2015, 04(02): 125-128.

目的

探讨ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉发生、发展中的作用。

方法

回顾性研究2012年3月至2014年3月在中山大学附属第一医院行胆囊切除术的60例患者临床资料。其中男31例，女29例；平均年龄（49±6）岁。所有患者均签署知情同意书，符合医学伦理学规定。根据病理学诊断结果，将患者分为胆囊胆固醇结石组(结石组)、胆囊胆固醇息肉组(息肉组)及正常胆囊切除对照组(对照组)。采用实时荧光定量PCR检测胆囊上皮细胞ABCG5和ABCG8 mRNA相对表达量。3组患者mRNA相对表达量的比较采用单因素方差分析和LSD-t检验。

结果

结石组ABCG5和ABCG8 mRNA相对表达量分别为3.3±0.9和6.9±1.5，明显高于对照组的2.4±0.6和4.3±1.5（LSD-t=23.58，16.55；P<0.05）。息肉组ABCG5和ABCG8 mRNA相对表达量分别为2.6±0.7和4.6±1.3，与对照组比较差异无统计学意义（LSD-t=1.18，0.73；P>0.05）。

结论

ABCG5和ABCG8基因可能通过不同机制在胆囊胆固醇结石和胆固醇息肉的发生、发展中发挥作用。

关键词: 胆囊结石病, 息肉, 胆固醇, ABCG5, ABCG8, 基因表达
Objective

To investigate ABCG5 and ABCG8 gene in the occurrence and development of cholesterol gallstone and cholesterol polyp.

Methods

Clinical data of 60 patients who underwent cholecystectomy in the First Affiliated Hospital of Sun Yat-sen University from March 2012 to March 2014 were studied retrospectively. Of the 60 patients, 31 were males and 29 were females with a mean age of (49±6) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. According to the results of pathological diagnosis, the patients were divided into the cholesterol gallstone group (gallstone group), the cholesterol polyp group (polyp group) and the routine cholecystectomy group (control group). The relative expression of ABCG5 and ABCG8 mRNA in gallbladder epithelial cells was determined by real-time fluorescence quantitative polymerase chain reaction (PCR). The comparison of the relative mRNA expression between three groups were conducted using One-way analysis of variance and LSD-t test.

Results

The relative expression of ABCG5 and ABCG8 mRNA was 3.3±0.9 and 6.9±1.5 respectively in gallstone group, which were significantly higher than 2.4±0.6 and 4.3±1.5 in control group (LSD-t=23.58, 16.55; P<0.05). The relative expression of ABCG5 and ABCG8 mRNA was 2.6±0.7 and 4.6±1.3 respectively in polyp group and no significant difference was observed when comparing with those in control group (LSD-t=1.18, 0.73; P>0.05).

Conclusion

ABCG5 and ABCG8 gene may play a role in the occurrence and development of cholesterol gallstone and cholesterol polyp through different mechanisms.

Key words: Cholecystolithiasis, Polyps, Cholesterol, ABCG5, ABCG8, Gene expression
表1 三组患者一般资料的比较
组别 例数 性别（例） 年龄（岁，±s） 体重指数（±s）
男 女
结石组 22 8 14 48±4 23±2
息肉组 23 13 10 48±6 20±1
对照组 15 10 5 50±4 20±1
统计值 ? χ2=0.125 F=3.43 F=4.73
P值 ? >0.05 >0.05 >0.05
表2 三组患者胆囊上皮细胞ABCG5及ABCG8 mRNA相对表达量的比较（±s）
组别 ABCG5mRNA ABCG8mRNA
结石组 3.3±0.9 6.9±1.5
息肉组 2.6±0.7 4.6±1.3
对照组 2.4±0.6 4.3±1.5
F值 10.36 7.58
P值 <0.05 <0.05
[1]
Stender S, Frikke-Schmidt R, Nordestgaard BG, et al. The ABCG5/8 cholesterol transporter and myocardial infarction versus gallstone disease[J]. J Am Coll Cardiol, 2014, 63(20):2121-2128.
[2]
von Kampen O, Buch S, Nothnagel M, et al. Genetic and functional identification of the likely causative variant for cholesterol gallstone disease at the ABCG5/8 lithogenic locus[J]. Hepatology, 2013, 57(6):2407-2417.
[3]
Hancock-Cerutti W, Rader DJ. Opposing effects of ABCG5/8 function on myocardial infarction and gallstone disease[J]. J Am Coll Cardiol, 2014, 63(20):2129-2130.
[4]
Cariati A, Piromalli E. Could omega-3 fatty acid prolonged intake reduce the incidence of symptomatic cholesterol gallstones disease?[J]. Clin Nutr, 2013, 32(3):486-487.
[5]
Marschall HU, Krawczyk M, Grunhage F, et al. Gallstone disease in Swedish twins is associated with the Gilbert variant of UGT1A1[J]. Liver Int, 2013, 33(6):904-908.
[6]
Simonsen MH, Erichsen R, Froslev T, et al. Postmenopausal estrogen therapy and risk of gallstone disease: a population-based case-control study[J]. Drug Saf, 2013, 36(12):1189-1197.
[7]
Di Ciaula A, Wang DQ, Garruti G, et al. Therapeutic reflections in cholesterol homeostasis and gallstone disease: a review[J]. Curr Med Chem, 2014, 21(12):1435-1447.
[8]
Wang C, Guo Y, Wang J, et al. Annexin A2 knockdown inhibits hepatoma cell growth and sensitizes hepatoma cells to 5-fluorouracil by regulating β-catenin and cyclin D1 expression[J]. Mol Med Rep, 2015, 11(3):2147-2152.
[9]
Ma KY, Yang N, Jiao R, et al. Dietary calcium decreases plasma cholesterol by down-regulation of intestinal Niemann-Pick C1 like 1 and microsomal triacylglycerol transport protein and up-regulation of CYP7A1 and ABCG 5/8 in hamsters[J]. Mol Nutr Food Res, 2011, 55(2):247-258.
[10]
Kerr ID, Haider AJ, Gelissen IC. The ABCG family of membrane-associated transporters: you don't have to be big to be mighty[J]. Br J Pharmacol, 2011, 164(7):1767-1779.
[11]
Gao X, Gu H, Li G, et al. Identification of an amino acid residue in ATP-binding cassette transport G1 critical for mediating cholesterol efflux[J]. Biochim Biophys Acta, 2012, 1821(3):552-559.
[12]
Pandey S. Genetic basis of gall stone disease[J]. Hepatology, 2013, 58(4):1519.
[13]
Liang Y, Chen J, Zuo Y, et al. Blueberry anthocyanins at doses of 0.5 and 1 % lowered plasma cholesterol by increasing fecal excretion of acidic and neutral sterols in hamsters fed a cholesterol-enriched diet[J]. Eur J Nutr, 2013, 52(3):869-875.
[14]
Ghanbari-Niaki A, Rahmati-Ahmadabad S, Zare-Kookandeh N. ABCG8 gene responses to 8 weeks treadmill running with or without Pistachia Atlantica (Baneh) extraction in female rats[J]. Int J Endocrinol Metab, 2012, 10(4):604-610.
[15]
Wang F, Li G, Gu HM, et al. Characterization of the role of a highly conserved sequence in ATP binding cassette transporter G (ABCG) family in ABCG1 stability, oligomerization, and trafficking[J]. Biochemistry, 2013, 52(52):9497-9509.
[16]
Ghanbari-Niaki A, Zare-Kookandeh N, Zare-Kookandeh A. ABCG5 gene responses to treadmill running with or without administration of Pistachio atlantica in female rats[J]. Iran J Basic Med Sci, 2014, 17(3):162-171.
[17]
Hirschfield GM, Chapman RW, Karlsen TH, et al. The genetics of complex cholestatic disorders[J]. Gastroenterology, 2013, 144(7):1357-1374.
[18]
Jakulj L, Vissers MN, Tanck MW, et al. ABCG5/G8 polymorphisms and markers of cholesterol metabolism: systematic review and meta-analysis[J]. J Lipid Res, 2010, 51(10):3016-3023.
[1] 朱连华, 费翔, 韩鹏, 姜波, 李楠, 罗渝昆. 高帧频超声造影在胆囊息肉样病变中的鉴别诊断价值[J]. 中华医学超声杂志（电子版）, 2023, 20(09): 904-910.
[2] 袁成雪, 张宗霞, 许婷, 斯郎拉姆. 三种内镜手术治疗结肠息肉的效果及安全性观察[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 78-81.
[3] 杨雪, 张伟, 尚培中, 宋创业, 尚丹丹, 张蔚. 胆囊十二指肠瘘结石经瘘口排出后自愈一例报道[J]. 中华普外科手术学杂志(电子版), 2023, 17(06): 707-708.
[4] 张建波, 东爱华. 不同腹腔镜手术治疗胆囊结石合并胆总管结石的疗效及并发症对比[J]. 中华普外科手术学杂志(电子版), 2023, 17(06): 693-696.
[5] 牛朝, 李波, 张万福, 靳文帝, 王春晓, 李晓刚. 腹腔镜袖状胃切除联合胆囊切除治疗肥胖合并胆囊结石安全性和疗效[J]. 中华肝脏外科手术学电子杂志, 2023, 12(06): 635-639.
[6] 周标, 陈达伟, 汤晓东, 陈胜, 刘双海, 邓志成. 腹腔镜下经胆囊管汇入部微切开取石在细径胆总管结石合并胆囊结石中的应用[J]. 中华肝脏外科手术学电子杂志, 2023, 12(04): 422-426.
[7] 陆乔友, 寸冬云, 虞弘, 郭鹏恒, 田大广. ICG荧光胆道造影在胆囊结石合并胆囊炎患者LC术中的应用[J]. 中华肝脏外科手术学电子杂志, 2023, 12(03): 284-288.
[8] 田广磊, 侯梅华, 李智德, 余波锋, 马俊杰, 李婷婷, 王春, 陈伦牮, 陈雄. 胆囊结石合并混合性神经内分泌-非神经内分泌肿瘤一例报告[J]. 中华肝脏外科手术学电子杂志, 2023, 12(03): 342-344.
[9] 张剑明, 叶文慧, 牟廷裕, 蓝孝亮, 邓海军. 腹腔镜全结直肠切除、回肠J型储袋-肛管吻合术近期并发症及防治策略[J]. 中华结直肠疾病电子杂志, 2023, 12(05): 388-395.
[10] 于迪, 于海波, 吴焕成, 李玉明, 苏彬, 陈馨. 发状分裂相关增强子1差异表达对胆固醇刺激下血管内皮细胞的影响[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(05): 264-270.
[11] 王飞飞, 王光林, 孟泽松, 李保坤, 曹龙飞, 张娟, 周超熙, 丁源一, 王贵英. 敲低IMPDH1对结肠癌SW480、HT29细胞生物功能的影响[J]. 中华临床医师杂志(电子版), 2023, 17(08): 884-890.
[12] 祁明花, 宋善兰, 俞丕超, 祁雁凌. 宫腔镜电切术联合生化汤加减治疗子宫内膜息肉的疗效及对短期复发的影响[J]. 中华临床医师杂志(电子版), 2023, 17(02): 171-175.
[13] 胡兵. 内镜下结直肠小息肉冷切术[J]. 中华胃肠内镜电子杂志, 2023, 10(03): 216-216.
[14] 中华医学会消化内镜学分会. 中国结直肠息肉冷切专家共识(2023年，杭州)[J]. 中华胃肠内镜电子杂志, 2023, 10(02): 73-82.
[15] 刘玉苓, 王婷婷, 吴高峰, 俞淑静. 健康体检人群内脏脂肪面积与新型炎症标志物的相关性研究[J]. 中华肥胖与代谢病电子杂志, 2023, 09(03): 197-202.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号