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中华肝脏外科手术学电子杂志 ›› 2015, Vol. 04 ›› Issue (02) : 125 -128. doi: 10.3877/cma.j.issn.2095-3232.2015.02.015

所属专题: 文献

基础研究

ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用
邓量1, 梁明懿2, 张昆松3,(), 许峰峰1, 黎文峰1, 张继红1   
  1. 1. 510080 广州,中山大学附属第一医院东院肝胆外科
    2. 510080 广州,中山大学附属第一医院妇产科
    3. 510080 广州,中山大学附属第一医院胆胰外科
  • 收稿日期:2014-10-19 出版日期:2015-04-10
  • 通信作者: 张昆松
  • 基金资助:
    广东省自然科学基金(S2012040006299,10451008901005954)

Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp

Liang Deng1, Mingyi Liang2, Kunsong Zhang3(), Fengfeng Xu1, Wenfeng Li1, Jihong Zhang1   

  1. 1. Department of Hepatobiliary Surgery, East Branch, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
    2. Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
    3. Department of Biliary Pancreatic Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
  • Received:2014-10-19 Published:2015-04-10
  • Corresponding author: Kunsong Zhang
  • About author:
    Corresponding author: Zhang Kunsong, Email:
引用本文:

邓量, 梁明懿, 张昆松, 许峰峰, 黎文峰, 张继红. ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用[J/OL]. 中华肝脏外科手术学电子杂志, 2015, 04(02): 125-128.

Liang Deng, Mingyi Liang, Kunsong Zhang, Fengfeng Xu, Wenfeng Li, Jihong Zhang. Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2015, 04(02): 125-128.

目的

探讨ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉发生、发展中的作用。

方法

回顾性研究2012年3月至2014年3月在中山大学附属第一医院行胆囊切除术的60例患者临床资料。其中男31例,女29例;平均年龄(49±6)岁。所有患者均签署知情同意书,符合医学伦理学规定。根据病理学诊断结果,将患者分为胆囊胆固醇结石组(结石组)、胆囊胆固醇息肉组(息肉组)及正常胆囊切除对照组(对照组)。采用实时荧光定量PCR检测胆囊上皮细胞ABCG5和ABCG8 mRNA相对表达量。3组患者mRNA相对表达量的比较采用单因素方差分析和LSD-t检验。

结果

结石组ABCG5和ABCG8 mRNA相对表达量分别为3.3±0.9和6.9±1.5,明显高于对照组的2.4±0.6和4.3±1.5(LSD-t=23.58,16.55;P<0.05)。息肉组ABCG5和ABCG8 mRNA相对表达量分别为2.6±0.7和4.6±1.3,与对照组比较差异无统计学意义(LSD-t=1.18,0.73;P>0.05)。

结论

ABCG5和ABCG8基因可能通过不同机制在胆囊胆固醇结石和胆固醇息肉的发生、发展中发挥作用。

Objective

To investigate ABCG5 and ABCG8 gene in the occurrence and development of cholesterol gallstone and cholesterol polyp.

Methods

Clinical data of 60 patients who underwent cholecystectomy in the First Affiliated Hospital of Sun Yat-sen University from March 2012 to March 2014 were studied retrospectively. Of the 60 patients, 31 were males and 29 were females with a mean age of (49±6) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. According to the results of pathological diagnosis, the patients were divided into the cholesterol gallstone group (gallstone group), the cholesterol polyp group (polyp group) and the routine cholecystectomy group (control group). The relative expression of ABCG5 and ABCG8 mRNA in gallbladder epithelial cells was determined by real-time fluorescence quantitative polymerase chain reaction (PCR). The comparison of the relative mRNA expression between three groups were conducted using One-way analysis of variance and LSD-t test.

Results

The relative expression of ABCG5 and ABCG8 mRNA was 3.3±0.9 and 6.9±1.5 respectively in gallstone group, which were significantly higher than 2.4±0.6 and 4.3±1.5 in control group (LSD-t=23.58, 16.55; P<0.05). The relative expression of ABCG5 and ABCG8 mRNA was 2.6±0.7 and 4.6±1.3 respectively in polyp group and no significant difference was observed when comparing with those in control group (LSD-t=1.18, 0.73; P>0.05).

Conclusion

ABCG5 and ABCG8 gene may play a role in the occurrence and development of cholesterol gallstone and cholesterol polyp through different mechanisms.

表1 三组患者一般资料的比较
表2 三组患者胆囊上皮细胞ABCG5及ABCG8 mRNA相对表达量的比较(±s
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