探讨蛋白磷酸酶1G（PPM1G）在肝细胞癌（肝癌）组织中的表达及其与患者预后的关系。

采用Oncomine和癌症基因组图谱（TCGA）公共数据集分析PPM1G在肝癌及癌旁组织中的表达及其与患者预后的关系。10对肝癌及配对癌旁组织标本来源于2018年5月至2021年4月安徽理工大学第一附属医院手术切除的10例肝癌患者。患者均签署知情同意书，符合医学伦理学规定。其中男7例，女3例；年龄57~68岁，中位年龄64岁。采用荧光定量PCR法及免疫组化验证肝癌PPM1G表达及其与预后的关系。两组PPM1G表达比较采用t检验。生存分析采用Kaplan-Meier法和Log-rank检验。

Oncomine数据库分析显示，PPM1G基因在肝癌中表达上调。TCGA数据库分析显示，肝癌组织PPM1G mRNA平均相对表达量为3.73±0.22，明显高于癌旁组织的3.45±0.11（t=2.99，P<0.05）；肝癌PPM1G低表达组患者总体生存率明高于肝癌PPM1G高表达组患者（HR=1.76，P<0.05）。荧光定量PCR分析显示，肝癌组织PPM1G mRNA相对表达量为4.46±0.38，明显高于癌旁组织的2.86±0.33（t=1.32，P<0.05）。免疫组化法检测显示，肝癌组织PPM1G高表达组PPM1G蛋白相对表达量为23.0±4.0，明显高于PPM1G低表达组的5.0±1.0（t=3.78，P<0.05）。生存分析显示，PPM1G高、低表达组中位生存期分别为32、41个月，PPM1G高表达组患者的总体生存率明显低于PPM1G低表达组患者（χ²=0.673，P<0.05）。

PPM1G在肝癌中高表达，PPM1G表达水平与肝癌患者的预后成负相关，其可能是肝癌预后评价的潜在指标。

To investigate the expression level of protein phosphatase 1G (PPM1G) in human hepatocellular carcinoma (HCC) and its relationship with the prognosis of HCC patients.

The expression level of PPM1G in HCC and adjacent tissues and its relationship with prognosis of HCC patients were analyzed by using Oncomine and The Cancer Genome Atlas (TCGA) databases. 10 pairs of HCC and matched adjacent tissues were collected from 10 HCC patients undergoing surgical resection in the First Affiliated Hospital of Anhui University of Science and Technology from May, 2018 to April, 2021. The informed consents of all patients were obtained and the local ethical committee approval was received. Among them, 7 patients were male and 3 female, aged from 57 to 68 years, with a median age of 64 years. The expression level of PPM1G in HCC and its relationship with clinical prognosis were investigated by quantitative fluorescence PCR and immunohistochemical staining. The expression levels of PPM1G between two groups were compared byt test. Survival analysis was performed Kaplan-Meier method and Log-rank test.

Oncomine database analysis showed that the expression level of PPM1G mRNA was up-regulated in HCC. TCGA database revealed that the average relative expression level of PPM1G mRNA was 3.73±0.22 in HCC, significantly higher than 3.45±0.11 in adjacent tissues (t=2.99, P<0.05). The overall survival rate of HCC patients with low PPM1G expression was significantly higher than that of those with high PPM1G expression (HR=1.76,P<0.05). Quantitative fluorescence PCR showed that, the relative expression level of PPM1G mRNA was 4.46±0.38 in HCC tissues, significantly higher than 2.86±0.33 in adjacent tissues (t=1.32, P<0.05). Immunohistochemical staining showed that, the relative expression level of the PPM1G protein was 23.0 ±4.0 with high PPM1G expression in HCC tissues, significantly higher than 5.0 ±1.0 with low PPM1G expression (t=3.78, P<0.05). Survival analysis showed that the median survival of patients with high and low PPM1G expression was 32 and 41 months. The overall survival rate of patients with high PPM1G expression was significantly lower than those with low PPM1G expression (χ²=0.673, P<0.05).

PPM1G is highly expressed in HCC. The expression level of PPM1G is negatively correlated with clinical prognosis of HCC patients, which may serve as a potential prognostic biomarker for HCC patients.