荧光腹腔镜反染法右前叶切除术是一种新兴的手术方法，已被证实在肝癌根治性手术切除治疗中安全有效。近年来，腹腔镜肝切除术迅速发展，并且荧光腹腔镜反染法作为一种新兴技术，通过荧光标记提高了手术的精确性和安全性，两者结合，在肝右前叶肿瘤切除上有明显优势，可避免常规术式对肿瘤的牵扯挤压及肿瘤破裂出血导致的血行转移，符合微创原则。然而，腹腔镜肝右前叶切除在肝实质劈离过程中，出血不易控制，并且由于腹腔镜视野较局限，使离断肝实质时容易丢失方向。因此本文将着重介绍荧光腹腔镜反染法右前叶切除术的方法及其优点和局限性，以期为临床手术提供参考。

Fluorescence-guided laparoscopic resection of the right anterior lobe using negative staining is an emerging surgical approach, which has been proven to be safe and efficacious in radical resection of liver cancer. In recent years, laparoscopic hepatectomy has developed rapidly. As a novel method, fluorescence-guided laparoscopic hepatectomy using negative staining improves surgical precision and safety through fluorescent labeling. The combination of these two methods has significant advantages in tumor resection of the right anterior lobe, avoiding the traction and compression of tumors by conventional surgery, preventing hematogenous metastasis caused by rupture and bleeding and conforming to minimally invasive principle. However, it is challenging to control the bleeding in the process of liver splitting during laparoscopic resection of the right anterior lobe. In addition, due to limited laparoscopic visual field, it is inclined to missing the direction during liver splitting. In this article, the advantages and limitations of fluorescence-guided laparoscopic resection of the right anterior lobe using negative staining were illustrated, aiming to provide reference for clinical surgery.