腹腔镜解剖性肝切除在肝脏良恶性疾病治疗中具有重要地位，但由于肝脏解剖结构复杂且缺乏明确解剖标志，术中精准确定切除平面仍面临重大挑战。本文基于Couinaud肝段解剖理论，系统探讨腹腔镜肝切除平面定位的理论基础与技术方法。通过肝蒂阻断寻找缺血线标记线及ICG荧光染色、结合肝静脉分支导向技术，建立多模态的肝切除平面定位体系。通过整合肝蒂阻断显示缺血线、ICG荧光染色和肝静脉导航三种技术，可显著提高腹腔镜解剖性肝切除的精准度和安全性。多技术融合的肝切除平面定位策略是实施精准腹腔镜解剖性肝切除的有效方法，值得临床推广应用。

Laparoscopic anatomical hepatectomy plays an important role in the treatment of benign and malignant liver diseases. However, due to complex anatomical structures of the liver and lack of clear anatomical landmarks, it remains a major challenge to precisely determine the resection plane intraoperatively. In this article, theoretical basis and technical methods of plane positioning during laparoscopic hepatectomy were systematically discussed based on Couinaud's liver segment classification. The precision and safety of laparoscopic anatomical hepatectomy can be significantly improved by integrating three technologies including hepatic pedicle occlusion, ICG fluorescence staining and hepatic vein navigation. Multi-technology integrated plane positioning strategy during hepatectomy is an effective approach to precisely implement laparoscopic anatomical hepatectomy, which is worthy of clinical application.