Protein kinase C-epsilon gene silenced by siRNA inhibits the development of cholangiocellular carcinoma and its mechanism

doi:10.3877/cma.j.issn.2095-3232.2014.06.012

Abstract

Abstract:

Objective

To investigate the role of protein kinase C-epsilon (PKCε) gene silenced by small interfering ribonucleic acid (siRNA) in inhibiting the development of cholangiocellular carcinoma and its mechanism.

Methods

Human cholangiocellular carcinoma QBC939 cells were respectively transfected using PKCε-siRNA and negative control (NC)-siRNA to establish PKC group and NC group. And untransfected control (CTRL) group was established. Cell counting kit (CCK)-8 assay was used to define the cell proliferation inhibition rate. Cell apoptosis rate was detected by flow cytometery. The expressions of protein PKCε and survivin were detected by Western blot. The comparison of three groups was conducted using one way analysis of variance and pairwise comparison using LSD-t test.

Results

The cell proliferation inhibition rates at 24, 48, 72 h [(7.52±0.33)%, (15.28±0.20)%, (37.12±0.45)% ] increased gradually in PKC group, where significant difference was observed compared with those in CTRL group (LSD-t=15.37, 27.12, 35.05; P<0.05). The cell apoptosis rate was (56.9±6.1)% in PKC group, which was significantly higher compared with that in CTRL group [(12.5±1.3) % ] (LSD-t=28.55, P<0.05). The expressions of protein PKCε and survivin decreased in PKC group compared with those in NC group and CTRL group.

Conclusion

PKCε gene silenced by siRNA may inhibit the development of cholangiocellular carcinoma through down-regulating the expression of protein survivin and promoting cell apoptosis.

Key words: Bile duct neoplasms, RNA interference, Protein kinase c-epsilon, Cell proliferation, Cell apoptosis

Mingming Guo, Jianghui Liu, Ruibin Cai, Jing Wang, Bin Chen. Protein kinase C-epsilon gene silenced by siRNA inhibits the development of cholangiocellular carcinoma and its mechanism[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2014, 03(06): 380-383.

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References 19

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