To investigate the influence of human cervical cancer gene 2 (HCCR-2) expression inhibited by siRNA on the proliferation of cholangiocarcinoma cells.

Pcmv6-AC-GFP/HCCR-2-siRNA lentiviral vector and Pcmv6-AC-GFP control vector were used to infect RBE human cholangiocarcinoma cells to establish the siRNA group and the control group. HCCR-2 protein expression was detected by Western blot. In vitro proliferation of cholangiocarcinoma cells was detected by MTT method and In vivo proliferation of cholangiocarcinoma cells was detected by mice subcutaneous implanted tumor model. The experimental data of two groups were compared using t test.

The average relative expression of HCCR-2 protein in RBE human cholangiocarcinoma cells of the siRNA group and the control group was respectively 0.21±0.03 and 0.70±0.02. Compared with the control group, the relative expression of HCCR-2 protein of the siRNA group was significantly reduced (t=-8.06, P<0.05). After 24 h and 48 h infection, the absorbance (A) measured in human cholangiocarcinoma cells of the siRNA group was respectively 0.05±0.01 and 0.16±0.01, which were significantly lower than 0.11±0.02 and 0.39±0.06 of the control group (t=-8.80, -11.31; P<0.05). By the 30^th day of subcutaneous implanted tumor grew, the tumor volume of the mice in the siRNA group was (106±28) mm³, which was significantly smaller than (516±24) mm³ of the mice in the control group (t=-29.80, P<0.05).

Expression of HCCR-2 silenced by siRNA may inhibit the proliferation of cholangiocarcinoma cells.