Construction of mouse model of liver implanted tumor after hepatectomy

doi:10.3877/cma.j.issn.2095-3232.2016.03.015

Abstract

Abstract:

Objective

To explore the construction of mouse model of liver implanted tumor after hepatectomy by implantation of liver tumor tissue.

Methods

H22 hepatocellular carcinoma (HCC) cells of mice were implanted to the scapula of 2 BALB/c (nu/nu) nude mice and tumors developed in 2 weeks. Eighteen nude mice were divided into three groups according to random number table method: non-hepatectomy group (group A), 30% hepatectomy group (group B) and 70% hepatectomy group (group C) with 6 mice in each group. The hepatectomy model of nude mice was established. The left lateral lobe and middle lobe were dissected for the nude mice in group A. The tissues of left lateral lobe were ligated and resected in group B. And the tissues of left lateralobe and middle lobe were ligated and resected in group C. The tumor tissues incised from the scapula were implanted to the livers of nude mice in three groups. The tumor formation rate and the volume of implanted tumors of three groups were compared. The tumor formation rate was compared using Fisher's exact probability test, and the volume of implanted tumors was compared using Kruskal-Wallis test and Bonferroni method.

Results

The tumor formation rate of group A, B and C after 2 weeks was respectively 6/6, 6/6 and 5/6, and no significant difference was observed (P=1.000). The median volume of implanted tumors in group A, B and C was respectively 19(6-33), 22(8-44) and 150(58-186) mm³. The median volume of implanted tumors in group C was significantly larger than those in group A and B (Z=9.03, 7.12; P<0.0167). The implanted tumors in three groups were all confirmed as HCC and with different degrees of necrosis and inflammatory cell infiltration by histopathological examination.

Conclusions

Implantation of liver tumor tissue can be used to construct an ideal mouse model of liver implanted tumor after major hepatectomy with the advantages of short experimental period, high tumor formation rate and good stability.

Key words: Hepatectomy, Carcinoma, hepatocellular, Neoplasm recurrence, local, Neoplasm seeding, Mice

Junbin Zhang, Genshu Wang, Jing Zhou, Zhiying Feng, Huiling Liu, Xiaolong Chen, Hui Li, Li Wang, Shihui Li, Jianxu Yang. Construction of mouse model of liver implanted tumor after hepatectomy[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2016, 05(03): 190-194.

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References 22

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