Establishment of rat models of reduced-size autologous liver transplantation

doi:10.3877/cma.j.issn.2095-3232.2021.04.018

Abstract

Abstract:

Objective

To investigate the establishment of rat models of reduced-size autologous liver transplantation.

Methods

After isoflurane inhalation anesthesia, 20 SD rats were treated with cold perfusion in situ of sodium lactate Ringer's solution containing heparin sodium (50 IU/ml) via the portal vein, and then the middle and left lateral liver lobes were resected to simulate the clinical process of autologous liver transplantation. Relevant parameters and survival of rats during the perioperative period were observed.

Results

Among the 20 rats receiving reduced-size autologous liver transplantation, 18 successfully survived.1 rat died of pneumothorax caused by diaphragm injury when separating the superior and inferior vena cava of the liver intraoperatively, and 1 rat died of hypothermia after postoperative resuscitation. The mean anesthesia induction time, operation time, anhepatic time, resected liver mass and postoperative recovery time from anesthesia were (1.9±0.3) min, (38.6±2.9) min, (16.0±0.9) min, (6.4±0.8) g and (3.1±0.3) min, respectively. The survival rate of rats at postoperative 1 week was 94%(17/18).

Conclusions

It is relatively convenient and reproducible to establish the rat models of reduced-size autologous liver transplantation, which yields high postoperative survival rate. This rat model effectively combines liver resection and orthotopic liver transplantation, which is suitable for the mechanism study of ischemia-reperfusion injury and autologous transplantation.

Key words: Liver transplantation, Transplantation, autologous, Models, animal, Rats

Tao Li, Apaer Shadike·, Jing Wu, Hao Zhang, Payiziwula Jiangduosi·, Qi Zeng, Anweier Nuerzhatijiang·, Tuxun Tuerhongjiang·. Establishment of rat models of reduced-size autologous liver transplantation[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2021, 10(04): 423-427.

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References 23

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