Establishment of a ferroptosis-related lncRNA prognostic model for patients with intrahepatic cholangiocarcinoma based on bioinformatics analysis

doi:10.3877/cma.j.issn.2095-3232.2023.02.012

Abstract

Abstract:

Objective

To establish a ferroptosis-related long non-coding RNA (lncRNA) prognostic model for patients with intrahepatic cholangiocarcinoma (ICC) based on bioinformatics analysis.

Methods

The gene expression data and clinical information of 36 patients with ICC and9 healthy counterparts were obtained from The Cancer Genome Atlas (TCGA) database, and the ferroptosis-related lncRNAs which were associated with clinical prognosis were extracted to establish the prognostic model. The predictive performance of ferroptosis-related lncRNA prognostic model was assessed by Cox's regression model and ROC curve. Survival analysis was carried out by Kaplan-Meier method andLog-rank test.

Results

Multivariate Cox's regression analysis revealed that two ferroptosis-related lncRNAs, AL161431.1 and LINC01587, were the independent prognostic factors of ICC (HR=2.99, 7.23; P<0.05). The lncRNA prognostic model was established for patients with ICC. The risk score =1.096×AL161431.1+1.979×LINC01587. Significant difference was observed in the overall survival between high-risk and low-risk groups by Kaplan-Meier survival curve (χ²=6.54, P<0.05). Multivariate Cox's regression analysis demonstrated that the risk score of ferroptosis-related lncRNA was an independent prognostic factor (HR=1.12, P<0.05). The area under time-dependent ROC curve (AUC) of the 1-, 2- and 3-year survival of this model was 0.883, 0.837 and 0.853, respectively. However, 1-year survival AUC of time-dependent ROC curve of tumor grading and staging was 0.598 and 0.637. The prognostic prediction ability of this model was significantly better than those of tumor grading and staging.

Conclusions

The prognostic model based on ferroptosis-related lncRNA can effectively predict the survival of patients with ICC.

Key words: Intrahepatic cholangiocarcinoma, Bile duct neoplasms, Ferroptosis, Long non-coding RNA, Computational biology

Jiantao Mo, Peize Yang, Ruiqi Cao, Qingyong Ma, Zheng Wang, Zheng Wu, Cancan Zhou. Establishment of a ferroptosis-related lncRNA prognostic model for patients with intrahepatic cholangiocarcinoma based on bioinformatics analysis[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(02): 185-189.

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