Fluorescence-guided laparoscopic resection of the right anterior lobe using negative staining is an emerging surgical approach, which has been proven to be safe and efficacious in radical resection of liver cancer. In recent years, laparoscopic hepatectomy has developed rapidly. As a novel method, fluorescence-guided laparoscopic hepatectomy using negative staining improves surgical precision and safety through fluorescent labeling. The combination of these two methods has significant advantages in tumor resection of the right anterior lobe, avoiding the traction and compression of tumors by conventional surgery, preventing hematogenous metastasis caused by rupture and bleeding and conforming to minimally invasive principle. However, it is challenging to control the bleeding in the process of liver splitting during laparoscopic resection of the right anterior lobe. In addition, due to limited laparoscopic visual field, it is inclined to missing the direction during liver splitting. In this article, the advantages and limitations of fluorescence-guided laparoscopic resection of the right anterior lobe using negative staining were illustrated, aiming to provide reference for clinical surgery.