Expression and significance of MMP-2 and collagen Ⅳ protein in hepatocellular carcinoma

doi:10.3877/cma.j.issn.2095-3232.2012.01.011

Abstract

Abstract:

Objective

To study the expression and significance of matrix metalloproteinase-2 (MMP-2) and collagen Ⅳ protein (collagen Ⅳ) in liver tissues of hepatocellular carcinoma (HCC) .

Methods

Forty HCC tissues were collected from the hepatic resection specieces in department of hepatic surgery in Guangdong General Hospital from Janaury 2005 to December 2007 and stained by immunohistochemistry to observe the expression of MMP-2 and collagen Ⅳ. Five normal liver tissues were obtained by autopsy as control. Local ethical committee approval had been received and that the informed concent of all participating subjects was obtained. The relationship between MMP-2, collagen Ⅳ expression and clinicopathologic features of HCC such as microvascular invasion, tumor diameter, tumor differentiation degree, recurrence was analyzed by test of Spearman’s rank-correlation coefficient.

Results

The positive expression of MMP-2 and collagen Ⅳ in HCC tissues were 29 cases(73%) and 38 cases(95%) respectively. The MMP-2 expression was negative in normal liver tissues, while the expression of collagen Ⅳ was observed in 3 samples among 5 tested normal liver tissues. The expressions of MMP-2 and collagen Ⅳ were related to the recurrence of HCC (all in P<0.05) . The positive expression rate of MMP-2 and collagen Ⅳ in HCC was markedly related to the microvascular invasion (all in P<0.05) .

Conclusions

The expressions of MMP-2 and collagen Ⅳ are positive in HCC tissues and may relate to the recurrence of hepatocellular carcinoma.

Key words: Hepatocellular carcinoma, Microvascular invasion, Matrix metalloproteinase-2, Collagen Ⅳ protein, Immunohistochemistry

Hui-ling WANG, Ying-liang OU, Zhi-xiang JIAN. Expression and significance of MMP-2 and collagen Ⅳ protein in hepatocellular carcinoma[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2012, 01(01): 46-50.

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Figures/Tables 8

References 14

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分组	例数	MMP-2			P值
分组	例数	(++)	(+)	(-)	P值
肝癌组	40	11	18	11	?
正常对照组	5	0	0	5	<0.01

分组	例数	MMP-2			P值
分组	例数	(++)	(+)	(-)	P值
肝癌组	40	11	18	11	?
正常对照组	5	0	0	5	<0.01

分组	例数	Ⅳ型胶原				P值
分组	例数	(+++)	(++)	(+)	(-)	P值
肝癌组	40	20	12	6	2	?
正常对照组	5	0	0	3	2	<0.01

分组	例数	Ⅳ型胶原				P值
分组	例数	(+++)	(++)	(+)	(-)	P值
肝癌组	40	20	12	6	2	?
正常对照组	5	0	0	3	2	<0.01

因素		例数	MMP-2			P值
因素		例数	(++)	(+)	(-)	P值
微血管浸润		?	?	?	?	<0.01
?	有浸润	16	9	6	1	?
?	无浸润	24	2	12	10	?
肿瘤直径		?	?	?	?	>0.05
?	<3 cm	4	1	2	1	?
?	3～5 cm	5	2	2	1	?
?	>5 cm	31	8	14	9	?
组织学分级		?	?	?	?	>0.05
?	高分化	8	1	2	5	?
?	中分化	23	8	12	3	?
?	低分化	9	2	4	3	?
复发情况		?	?	?	?	<0.05
?	有复发	20	8	10	2	?
?	无复发	20	3	8	9	?

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