Abstract:
Objective To investigate the optimum reperfusion duration of liver ischemia- reperfusion injury (IRI) model by occluding the proper hepatic artery in rats.
Methods Fourty-eight Sprague Dawley (SD) rats with mean weight of (200±25) g were randomly assigned to 8 groups by random number table method: IRI- 0, 1, 3, 6, 12, 24, 48 h and sham operation (SO) group with 6 rats in each group. The proper hepatic arteries in rats of IRI groups were selectively occluded for 1 h and then blood flow recovered. Samples of blood and liver tissues were collected at the time points of 0, 1, 3, 6, 12, 24, 48 h of reperfusion. In SO group, samples were collected after the proper hepatic artery was isolated and the first portal was exposed for 1 h. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), pathological changes, DNA fragmentation rates, and levels of Ki-67 expression of liver tissues were observed in each group. Measurement data of multiple groups were compared using one-way analysis of variance and LSD-t test.
Results The levels of serum ALT in IRI- 0, 1, 3, 6, 12, 24, 48 h and SO group were (53±25), (85±20), (96±18), (411±96), (87±19), (81±15), (46±6), (60±14) U/L respectively. The levels of serum AST were (238±63), (364±111), (375±68), (1 291±511), (800±87), (854±218), (484±219), (248±94) U/L accordingly. The levels of serum ALT, AST in IRI 6 h group were the highest (F=36.015, 18.241; P<0.05). The damage of liver tissues in IRI 6 h group was the most serious. The DNA fragmentation rates of liver tissues were (7.5±1.5)%, (9.2±2.2)%, (9.3±2.3)%, (12.6±2.4)%, (6.3±1.0)%, (5.4±0.9)%, (4.5±0.8)%, (4.5±1.1)% accordingly, which was the highest in IRI 6 h group (F=15.992, P<0.05). The levels of Ki-67 expression of liver tissues were (3.5±1.4), (5.6±1.8), (8.7±2.3), (13.7±2.4), (15.2±1.2), (20.5±2.2), (31.8±2.5), (2.4±1.2) / high power field accordingly, which was the highest in IRI 48 h group (F=261.707, P<0.05).
Conclusions The liver IRI model can be successfully established by occluding the proper hepatic artery, and the optimum reperfusion duration of IRI is 6 h.
Key words:
Hepatic artery,
Reperfusion injury,
Models, animal,
Apoptosis,
Cell proliferation
Ying﹡ Lin, Huiling Liu, Bing Wang, Lixian Zeng, Huixin He, Zhuofu Wen, Genshu Wang, Bin Wu. Research on the optimum reperfusion duration of liver ischemia-reperfusion injury model by occluding proper hepatic artery in rats[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2014, 03(01): 52-56.