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中华肝脏外科手术学电子杂志 ›› 2018, Vol. 07 ›› Issue (01) : 73 -76. doi: 10.3877/cma.j.issn.2095-3232.2018.01.018

所属专题: 文献

基础研究

载脂蛋白F在肝细胞癌患者生存预后中的临床意义
周伯宣1, 姚志成2, 熊志勇2, 李瑞曦1, 代天星1, 许明星1, 范伟明1, 周正1, 梁豪1, 邓美海1, 凌云彪1,()   
  1. 1. 510630 广州,中山大学附属第三医院肝胆外科
    2. 510630 广州,中山大学附属第三医院普通外科
  • 收稿日期:2017-11-07 出版日期:2018-02-10
  • 通信作者: 凌云彪
  • 基金资助:
    国家自然科学基金(81470860); 广东省科技计划项目(2012A020602002)

Clinical significance of apolipoprotein F in prognosis of patients with hepatocellular carcinoma

Boxuan Zhou1, Zhicheng Yao2, Zhiyong Xiong2, Ruixi Li1, Tianxing Dai1, Mingxing Xu1, Weiming Fan1, Zheng Zhou1, Hao Liang1, Meihai Deng1, Yunbiao Ling1,()   

  1. 1. Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    2. General Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2017-11-07 Published:2018-02-10
  • Corresponding author: Yunbiao Ling
  • About author:
    Corresponding author: Ling Yunbiao, Email:
引用本文:

周伯宣, 姚志成, 熊志勇, 李瑞曦, 代天星, 许明星, 范伟明, 周正, 梁豪, 邓美海, 凌云彪. 载脂蛋白F在肝细胞癌患者生存预后中的临床意义[J/OL]. 中华肝脏外科手术学电子杂志, 2018, 07(01): 73-76.

Boxuan Zhou, Zhicheng Yao, Zhiyong Xiong, Ruixi Li, Tianxing Dai, Mingxing Xu, Weiming Fan, Zheng Zhou, Hao Liang, Meihai Deng, Yunbiao Ling. Clinical significance of apolipoprotein F in prognosis of patients with hepatocellular carcinoma[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2018, 07(01): 73-76.

目的

探讨载脂蛋白(Apo)F在肝细胞癌(肝癌)中的表达及其在患者生存预后中的应用价值。

方法

50例肝癌标本取自2015年9月至2016年9月在中山大学附属第三医院手术切除患者,且均经术后病理学检查证实。患者均签署知情同意书,符合医学伦理学规定。其中男37例,女13例;年龄31~67岁,中位年龄53岁。采用RT-PCR法检测肝癌组织ApoF mRNA的表达情况。利用基因表达汇编(GEO)数据库,对表达谱资料进行分析。两组ApoF表达量比较采用t检验,临床相关指标的相关性分析采用χ2检验,生存预后分析采用Kaplan-Meier法和Log rank检验。

结果

肝癌组织ApoF mRNA平均相对表达量为0.15±0.07,明显低于癌旁组织的0.55±0.09(t=-6.26,P<0.05)。GEO在线分析显示ApoF表达与患者肝硬化状态明显相关,合并肝硬化的肝癌患者多伴有ApoF低表达(χ2=4.626,P<0.05)。ApoF高表达组和低表达组患者5年无瘤生存率分别为55.9%、32.0%,差异有统计学意义(χ2=3.939,P<0.05)。

结论

肝癌组织中存在ApoF低表达,ApoF低表达与患者肝硬化状态有关。ApoF低表达患者预后较差,ApoF具有抑癌作用。

Objective

To investigate the expression of apolipoprotein (Apo) F in hepatocellular carcinoma (HCC) and its application value in the prognosis of patients with HCC.

Methods

50 HCC samples were procured from patients undergoing surgical resection in the Third Affiliated Hospital of Sun Yat-sen University between September 2015 and September 2016, and all the samples were confirmed by postoperative pathological examination. The informed consents of all patients were obtained and the local ethical committee approval was received. There were 37 males and 13 females, aged from 31-67 with a median age of 53 years old. The expression of ApoF mRNA in HCC tissues was detected by RT-PCR. The expression profile was analyzed by using data from the Gene Expression Omnibus (GEO). The expression of ApoF between two groups were compared by t test. Correlation analysis of clinical related parameter was conducted by Chi-square test, and survival prognosis was analyzed by Kaplan-Meier test and Log rank test.

Results

The average relative expression of ApoF mRNA in HCC tissues was 0.15±0.07, significantly lower than 0.55±0.09 in the adjacent tissues (t=-6.26, P<0.05). GEO online analysis showed that expression of ApoF was significantly correlated with the status of liver cirrhosis, and most HCC patients with liver cirrhosis presented low expression of ApoF (χ2=4.626, P<0.05). The 5-year disease-free survival was respectively 55.9% and 32.0% in ApoF high expression group and low expression group, where significant difference was observed (χ2=3.939, P<0.05).

Conclusions

Low expression of ApoF exists in HCC tissues, and it is related to the liver cirrhosis status of patients. Patients with low ApoF expression present poorer prognosis. ApoF plays a role in inhibiting the cancer.

表1 ApoF表达水平与肝癌患者临床病理学参数关系
图1 ApoF高、低表达组肝癌患者生存预后Kaplan-Meier生存曲线图
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