探讨一种改良可复性梗阻性黄疸大鼠模型建立的可行性，并评价其应用价值。

90只SD大鼠按随机数字表法随机分为改良组、对照组和假手术组，每组30只。3组分别建立改良型大鼠可复性梗阻性黄疸模型、传统可复性黄疸模型和假手术；术后7 d解除胆管梗阻。3组围手术期肝功能比较采用单因素方差分析和LSD-t检验，率的比较采用χ²检验。

解除梗阻后改良组死于胆漏2只，对照组9只，差异有统计学意义（χ²=4.01，P<0.05）。改良组与对照组从术后至再通7 d的TB、ALT、ALP水平和肝脏组织学改变呈现相似的变化规律。改良组再通后3 d的TB、ALT、ALP分别为（4.3±1.5）μmol/L、（25±8）U/L、（130±14）U/L，明显低于对照组的（23.5±3.6）μmol/L、（49±8）U/L、（170±14）U/L（LSD-t=-23.09，-10.36，-9.67；P<0.05），且改良组表现出轻度淤胆、胆管上皮正常、管壁稍有增生，而对照组中度淤胆、胆管内见狭窄。

改良可复性梗阻性黄疸大鼠模型对胆管损伤较传统模型轻微，更好地模拟了临床梗阻性黄疸再通后恢复胆肠循环的生理过程，是一种安全、简便、经济的动物模型。

To explore the feasibility of establishing a modified rat model of reversible obstructive jaundice and to assess its value.

90 SD rats were randomly divided into the modified, control and sham operation groups (30 rats in each group) according to random number table method. The modified rat model of reversible obstructive jaundice, traditional model of reversible jaundice and sham operation models were established in 3 groups. The bile duct obstruction was relieved at 7 d after operation. The perioperative liver function in 3 groups was compared by one-way ANOVA and LSD-t test. The ratio comparison was performed by Chi-square test.

After the obstruction was relieved, 2 rats died of bile leakage in modified group and 9 in control group, where significance difference was observed (χ²=4.01, P<0.05). From the end of surgery to the 7^th day after recanalization, TB, ALT, ALP levels and liver histology were observed with similar changes between the modified and control groups. At 3 d after recanalization, TB, ALT and ALP levels in modified group were (4.3±1.5) μmol/L, (25±8) U/L and (130±14) U/L, significantly lower than (23.5±3.6) μmol/L, (49±8) U/L and (170±14) U/L in control group (LSD-t=-23.09, -10.36, -9.67; P<0.05). Mild cholestasis, normal bile duct epithelium and slight hyperplasia of the bile duct wall were observed in modified group, whereas moderate cholestasis and intraductal stenosis were noted in control group.

Compared with the traditional model, the modified rat model of reversible obstructive jaundice does less injury to bile duct and better mimics the physiological process of recanalization of obstructive jaundice and restoration of bile-intestinal circulation, which is a safe, simple and economical animal model.