miR-122与结直肠癌术后肝转移的关系

doi:10.3877/cma.j.issn.2095-3232.2020.04.020

目的

探讨miR-122与结直肠癌术后肝转移的关系。

方法

回顾性分析2008年1月至2014年1月中国人民解放军联勤保障部队第909医院收治的186例结直肠癌术后肝转移患者临床资料。患者均签署知情同意书，符合医学伦理学规定。其中男112例，女74例；年龄33～76岁，中位年龄52岁；左半结肠癌43例，右半结肠癌79例，直肠癌64例。采用qRT-PCR检测癌组织和癌旁组织中miR-122的相对表达量。两组miR-122相对表达量比较采用t检验。miR-122表达与术后肝转移时间关系采用线性相关分析。

结果

结直肠癌组织miR-122平均相对表达量为1.28±0.14，明显高于癌旁组织的0.83±0.09（t=124.00，P<0.05）。癌组织miR-122相对表达量与结直肠癌术后肝转移患者肿瘤直径、肿瘤分化、淋巴结转移相关（t=-7.17，4.16，-8.69；P<0.05）。结直肠癌术后肝转移时间为（23±9）个月。癌组织miR-122相对表达量与结直肠癌术后肝转移时间成负相关（r=-0.67，P<0.05）。

结论

结直肠癌组织miR-122高表达。miR-122表达量与术后肝转移时间成负相关，其表达量越高肝转移越早。

关键词: 结直肠肿瘤, 肿瘤转移, 微RNAs

Objective

To explore the correlation between miR-122 and postoperative colorectal liver metastases.

Methods

Clinical data of 186 patients with postoperative liver metastases from colorectal cancer who were admitted in the 909^th Hospital of Joint Logistics Support Force of PLA from January 2008 to January 2014 were retrospectively analyzed. The informed consents of all patients were obtained and the local ethical committee approval was received. Among them, 112 patients were male and 74 female, aged 33-76 years old and with a median age of 52. 43 cases were with left-sided colon cancer, 79 cases with right-sided colon cancer and 64 cases with rectal cancer. The relative expression of miR-122 in cancerous and paraneoplastic tissues was quantified by qRT-PCR. Comparison of miR-122 relative expression between two groups was performed using t test. The correlation between miR-122 expression and time to postoperative liver metastasis was analyzed by linear correlation analysis.

Results

The average relative expression of miR-122 in colorectal cancer tissues was 1.28±0.14, which was significantly higher than 0.83±0.09 in paraneoplastic tissues (t=124.00, P<0.05). miR-122 relative expression was associated with tumor diameter, tumor differentiation, and lymph node metastasis in patients with postoperative liver metastases from colorectal cancer (t=-7.17, 4.16, -8.69; P<0.05). Time to postoperative liver metastasis from colorectal cancer was (23±9) months. The miR-122 relative expression in cancer tissues was negatively correlated with the time to postoperative liver metastasis (r=-0.67, P<0.05).

Conclusions

miR-122 is highly expressed in colorectal cancer tissue. The miR-122 expression was negatively correlated with the time to postoperative liver metastasis. The higher expression is, the earlier liver metastasis occurs.

Key words: Colorectal neoplasms, Neoplasm metastasis, MicroRNAs

表1 癌组织中miR-122相对表达量与结直肠癌术后肝转移患者临床病理学因素的关系（±s）

因素		例数	miR-122相对表达量	t值	P值
性别		?	?	-0.80	0.44
?	男	112	1.27±0.12	?	?
?	女	74	1.29±0.13	?	?
年龄		?	?	0.46	0.65
?	<55岁	95	1.28±0.14	?	?
?	≥55岁	91	1.27±0.11	?	?
肿瘤直径		?	?	-7.17	<0.05
?	<4 cm	85	1.21±0.12	?	?
?	≥4 cm	101	1.33±0.10	?	?
肿瘤分化		?	?	4.16	<0.05
?	低分化、未分化	102	1.32±0.11	?	?
?	中、高分化	84	1.24±0.13	?	?
淋巴结转移		?	?	-8.69	<0.05
?	无	72	1.19±0.11	?	?
?	有	114	1.33±0.09	?	?

表1 癌组织中miR-122相对表达量与结直肠癌术后肝转移患者临床病理学因素的关系（±s）

因素		例数	miR-122相对表达量	t值	P值
性别		?	?	-0.80	0.44
?	男	112	1.27±0.12	?	?
?	女	74	1.29±0.13	?	?
年龄		?	?	0.46	0.65
?	<55岁	95	1.28±0.14	?	?
?	≥55岁	91	1.27±0.11	?	?
肿瘤直径		?	?	-7.17	<0.05
?	<4 cm	85	1.21±0.12	?	?
?	≥4 cm	101	1.33±0.10	?	?
肿瘤分化		?	?	4.16	<0.05
?	低分化、未分化	102	1.32±0.11	?	?
?	中、高分化	84	1.24±0.13	?	?
淋巴结转移		?	?	-8.69	<0.05
?	无	72	1.19±0.11	?	?
?	有	114	1.33±0.09	?	?

图1 癌组织miR-122表达与结直肠癌肝转移时间的相关性分析

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Correlation between miR-122 and postoperative colorectal liver metastases

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Correlation between miR-122 and postoperative colorectal liver metastases