切换至 "中华医学电子期刊资源库"

中华肝脏外科手术学电子杂志 ›› 2021, Vol. 10 ›› Issue (01) : 98 -103. doi: 10.3877/cma.j.issn.2095-3232.2021.01.021

所属专题: 文献

基础研究

基于生物信息学检测TRIM47基因在肝癌中的表达及意义
伍隽华1,(), 顾娇娇2, 陈云扬1, 余杰雄1, 邝乃乐1, 黄皓川1   
  1. 1. 529000 广东省江门市中心医院肝胆外科
    2. 510630 广州,中山大学附属第三医院肝胆外科
  • 收稿日期:2020-10-20 出版日期:2021-02-10
  • 通信作者: 伍隽华
  • 基金资助:
    2019年度江门市中心医院科研启动基金资助项目(D201902)

Expression of TRIM47 and its significance in liver cancer base on bioinformatic test

Juanhua Wu1,(), Jiaojiao Gu2, Yunyang Chen1, Jiexiong Yu1, Naile Kuang1, Haochuan Huang1   

  1. 1. Department of Hepatobiliary Surgery, Jiangmen Central Hospital, Jiangmen 529000, China
    2. Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2020-10-20 Published:2021-02-10
  • Corresponding author: Juanhua Wu
引用本文:

伍隽华, 顾娇娇, 陈云扬, 余杰雄, 邝乃乐, 黄皓川. 基于生物信息学检测TRIM47基因在肝癌中的表达及意义[J/OL]. 中华肝脏外科手术学电子杂志, 2021, 10(01): 98-103.

Juanhua Wu, Jiaojiao Gu, Yunyang Chen, Jiexiong Yu, Naile Kuang, Haochuan Huang. Expression of TRIM47 and its significance in liver cancer base on bioinformatic test[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2021, 10(01): 98-103.

目的

探讨三结构域蛋白47(TRIM47)基因在肝癌中的表达及其临床意义。

方法

通过生物信息学方法,利用UALCAN、HPA数据库分析TRIM47在肝癌中的基因表达和蛋白表达。利用Kaplan-Meier生存数据库分析肝癌患者总生存率,评估TRIM47在肝癌患者预后中的价值。利用cBioPortal数据库分析肝癌患者中TRIM47的基因突变频率,以及TRIM47基因突变对肝癌患者总生存率的影响。构建稳定表达TRIM47的肝癌细胞株Hep3B、Huh-7,采用qRT-PCR检测肝癌细胞TRIM47 mRNA,CCK-8法检测其细胞增殖能力。肝癌TRIM47 mRNA相对表达量和增殖能力比较采用秩和检验或t检验。

结果

UALCAN数据库分析显示肝癌组织中TRIM47 mRNA相对表达量中位数为18.95(10.79,31.81),明显高于正常肝组织中的7.09(5.76,9.61) (P<0.05)。HPA数据库分析显示肝癌组织中TRIM47蛋白水平呈高表达,而在正常肝组织中呈低表达。UALCAN数据库分析显示随着肿瘤组织中TRIM47表达水平的升高,患者临床分期、肿瘤分级越差(P<0.05)。Kaplan-Meier生存数据库分析显示低表达TRIM47的肝癌患者总体生存期更长(HR=1.59,95%CI:1.12~2.26;P<0.05)。cBioPortal数据库分析显示TRIM47在肝癌患者中的基因突变率可达14%,且能明显影响患者总生存率(P=0.013 6)。Hep3B-TRIM47和Huh-7-TRIM47肝癌细胞TRIM47 mRNA平均相对表达量分别为(4.87±0.14)×10-3、(2.47±0.47)×10-3,明显高于对照组的(0.60±0.08)×10-3、(0.18±0.05)×10-3t=26.560,4.856;P<0.05)。Hep3B-TRIM47和Huh-7-TRIM47肝癌细胞株增殖能力显著增强。

结论

TRIM47基因可通过促进肝癌细胞增殖影响肝癌患者的预后,TRIM47可能成为预测肝癌患者预后的一种新型标记物。

Objective

To investigate the expression and clinical significance of tripartite motif-containing 47 (TRIM-47) gene in liver cancer.

Methods

The expression of TRIM47 mRNA and protein in liver cancer were analyzed by bioinformatics methods using UALCAN and HPA databases. The overall survival of liver cancer patients was analyzed by Kaplan-Meier survival database. The prognostic value of TRIM47 in liver cancer patients was evaluated. The frequency of TRIM47 gene mutation was analyzed by cBioPortal database. The effect of TRIM47 gene mutation on the overall survival of liver cancer patients was assessed. The liver cancer cell lines Hep3B and Huh-7 which stably expressed TRIM47 were constructed. The expression of TRIM47 mRNA in liver cancer cells was detected by qRT-PCR. The cell proliferation ability was detected by CCK-8 assay. The relative expression of TRIM47 mRNA and the proliferation ability of liver cancer cells were compared by rank-sum test or t test.

Results

UALCAN database analysis showed that the median relative expression of TRIM47 mRNA in liver cancer tissues was 18.95(10.79, 31.81), significantly higher than 7.09(5.76, 9.61) in normal liver tissues (P<0.05). HPA database analysis demonstrated that TRIM47 protein highly expressed in liver cancer tissues, whereas lowly expressed in normal liver tissues. UALCAN database revealed that with the up-regulation of TRIM47 expression in tumor tissues, the clinical stage and tumor grade became significantly worse (both P<0.05). Kaplan-Meier survival database analysis showed that the overall survival of liver cancer patients with low expression of TRIM47 was significantly longer (HR=1.59, 95%CI: 1.12-2.26; P<0.05). cBioPortal database analysis indicated that the mutation rate of TRIM47 gene in patients with liver cancer reached up to 14%, and significantly affected the overall survival (P=0.013 6). The mean relative expression levels of TRIM47 mRNA in Hep3B-TRIM47 and Huh-7-TRIM47 liver cancer cells were (4.87±0.14)×10-3 and (2.47±0.47)×10-3, significantly higher than (0.60±0.08)×10-3 and (0.18±0.05)×10-3 in the control group (t=26.560, 4.856; P<0.05). The proliferation ability of Hep3B-TRIM47 and Huh-7-TRIM47 cell lines was significantly enhanced.

Conclusions

TRIM47 can affect the clinical prognosis of patients with liver cancer by promoting the proliferation of liver cancer cells. TRIM47 may become a novel biomarker for predicting the prognosis of liver cancer patients.

图1 不同数据库肝癌组织中TRIM47的表达谱
图2 UALCAN数据库分析TRIM47表达水平与肝癌患者临床特征之间的关系
图3 TRIM47在肝癌中的突变情况及对患者生存的影响
图4 TRIM47过表达组与对照组细胞生长曲线
[1]
Yang JD, Hainaut P, Gores GJ, et al. A global view of hepatocellular carcinoma: trends, risk, prevention and management[J]. Nat Rev Gastroenterol Hepatol, 2019, 16(10):589-604.
[2]
Li C, Li R, Zhang W. Progress in non-invasive detection of liver fibrosis[J]. Cancer Biol Med, 2018, 15(2):124-136.
[3]
Forner A, Reig M, Bruix J. Hepatocellular carcinoma[J]. Lancet, 2018, 391(10127):1301-1314.
[4]
Dawkins J, Webster RM. The hepatocellular carcinoma market[J]. Nat Rev Drug Discov, 2019, 18(1):13-14.
[5]
Hatakeyama S. TRIM family proteins: roles in autophagy, immunity, and carcinogenesis[J]. Trends Biochem Sci, 2017, 42(4):297-311.
[6]
Hatakeyama S. TRIM proteins and cancer[J]. Nat Rev Cancer, 2011, 11(11):792-804.
[7]
Fujimura T, Inoue S, Urano T, et al. Increased expression of tripartite motif (TRIM) 47 is a negative prognostic predictor in human prostate cancer[J]. Clin Genitourin Cancer, 2016, 14(4):298-303.
[8]
Li W, Zhang W, Deng W, et al. Quantitative proteomic analysis of mitochondrial proteins differentially expressed between small cell lung cancer cells and normal human bronchial epithelial cells[J]. Thorac Cancer, 2018, 9(11):1366-1375.
[9]
Liang Q, Tang C, Tang M, et al. TRIM47 is up-regulated in colorectal cancer, promoting ubiquitination and degradation of SMAD4[J].J Exp Clin Cancer Res, 2019, 38(1):159.
[10]
Chandrashekar DS, Bashel B, Balasubramanya SAH, et al. UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses[J]. Neoplasia, 2017, 19(8):649-658.
[11]
Asplund A, Edqvist PH, Schwenk JM, et al. Antibodies for profiling the human proteome-the human protein Atlas as a resource for cancer research[J]. Proteomics, 2012, 12(13):2067-2077.
[12]
Hou GX, Liu P, Yang J, et al. Mining expression and prognosis of topoisomerase isoforms in non-small-cell lung cancer by using Oncomine and Kaplan-Meier plotter[J]. PLoS One, 2017, 12(3):e0174515.
[13]
Popovic D, Vucic D, Dikic I. Ubiquitination in disease pathogenesis and treatment[J]. Nat Med, 2014, 20(11):1242-1253.
[14]
Micale L, Chaignat E, Fusco C, et al. The tripartite motif: structure and function[J]. Adv Exp Med Biol, 2012(770):11-25.
[15]
Cambiaghi V, Giuliani V, Lombardi S, et al. TRIM proteins in cancer[J]. Adv Exp Med Biol, 2012(770):77-91.
[16]
Zhou Z, Liu Y, Ma M, et al. Knockdown of TRIM44 inhibits the proliferation and invasion in papillary thyroid cancer cells through suppressing the Wnt/β-catenin signaling pathway[J]. Biomed Pharmacother, 2017(96):98-103.
[17]
Huang XQ, Zhang XF, Xia JH, et al. Tripartite motif-containing3 (TRIM3) inhibits tumor growth and metastasis of liver cancer[J]. Chin J Cancer, 2017, 36(1):77.
[18]
Li L, Dong L, Qu X, et al. Tripartite motif 16 inhibits hepatocellular carcinoma cell migration and invasion[J]. Int J Oncol, 2016, 48(4): 1639-1649.
[19]
Han Y, Tian H, Chen P, et al. TRIM47 overexpression is a poor prognostic factor and contributes to carcinogenesis in non-small cell lung carcinoma[J]. Oncotarget, 2017, 8(14):22730-22740.
[1] 汪洪斌, 张红霞, 何文, 杜丽娟, 程令刚, 张雨康, 张萌. 低级别阑尾黏液性肿瘤与阑尾黏液腺癌超声及超声造影特征分析[J/OL]. 中华医学超声杂志(电子版), 2024, 21(09): 865-871.
[2] 史学兵, 谢迎东, 谢霓, 徐超丽, 杨斌, 孙帼. 声辐射力弹性成像对不可切除肝细胞癌门静脉癌栓患者放射治疗效果的评价[J/OL]. 中华医学超声杂志(电子版), 2024, 21(08): 778-784.
[3] 燕速, 霍博文. 腹腔镜食管胃结合部腺癌根治性切除术[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 13-13.
[4] 李子禹, 卢信星, 李双喜, 陕飞. 食管胃结合部腺癌腹腔镜手术重建方式的选择[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 5-8.
[5] 李乐平, 张荣华, 商亮. 腹腔镜食管胃结合部腺癌根治淋巴结清扫策略[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 9-12.
[6] 李刘庆, 陈小翔, 吕成余. 全腹腔镜与腹腔镜辅助远端胃癌根治术治疗进展期胃癌的近中期随访比较[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 23-26.
[7] 李华志, 曹广, 刘殿刚, 张雅静. 不同入路下行肝切除术治疗原发性肝细胞癌的临床对比[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 52-55.
[8] 常小伟, 蔡瑜, 赵志勇, 张伟. 高强度聚焦超声消融术联合肝动脉化疗栓塞术治疗原发性肝细胞癌的效果及安全性分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 56-59.
[9] 冯旺, 马振中, 汤林花. CT扫描三维重建在肝内胆管细胞癌腹腔镜肝切除术中的临床研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 104-107.
[10] 陈浩, 王萌. 胃印戒细胞癌的临床病理特征及治疗选择的研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 108-111.
[11] 郭兵, 王万里, 何凯, 黄汉生. 腹腔镜下肝门部胆管癌根治术[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(01): 143-143.
[12] 魏丽霞, 张安澜, 周宝勇, 李明. 腹腔镜下Ⅲb型肝门部胆管癌根治术[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(01): 145-145.
[13] 王湛, 李文坤, 杨奕, 徐芳, 周敏思, 苏珈仪, 王亚丹, 吴静. 炎症指标在早发性结直肠肿瘤中的应用[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 802-810.
[14] 刘琦, 王守凯, 王帅, 苏雨晴, 马壮, 陈海军, 司丕蕾. 乳腺癌肿瘤内微生物组的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 841-845.
[15] 广东省护士协会介入护士分会, 广东省医师协会介入医师分会. 原发性肝癌低血糖患者护理规范管理专家共识[J/OL]. 中华临床医师杂志(电子版), 2024, 18(08): 709-714.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?