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中华肝脏外科手术学电子杂志 ›› 2023, Vol. 12 ›› Issue (05) : 499 -503. doi: 10.3877/cma.j.issn.2095-3232.2023.05.006

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原发性肝癌的分子诊断
王楚风, 蒋安()   
  1. 710004 西安交通大学第二附属医院移植外科
  • 收稿日期:2023-07-10 出版日期:2023-10-10
  • 通信作者: 蒋安
  • 基金资助:
    陕西省重点研发计划(2022SF-372)

Molecular diagnosis of primary liver cancer

Chufeng Wang, An Jiang()   

  • Received:2023-07-10 Published:2023-10-10
  • Corresponding author: An Jiang
引用本文:

王楚风, 蒋安. 原发性肝癌的分子诊断[J/OL]. 中华肝脏外科手术学电子杂志, 2023, 12(05): 499-503.

Chufeng Wang, An Jiang. Molecular diagnosis of primary liver cancer[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(05): 499-503.

原发性肝癌(肝癌)发病率呈现上升趋势,病死率位居全球第4位[1,2]。肝癌具有起病隐匿、进展速度快、异质性强、病理机制复杂、预后差等特点,我国肝癌发生率及病死率均超过全球平均水平的50%,发现时往往处于晚期,失去手术机会。因此,探索肝癌发生的分子机制、针对肝癌的早发现、早筛查、早诊断对于提高肝癌患者的生存率和生活质量有重要意义。肝穿刺活检虽然是肝癌确诊的金标准,但面对分化程度较好的肝癌组织时容易误诊。另外,患者的依从性以及肝穿刺可能发生并发症也决定了肝穿刺活检并不是肝癌早期诊断的首选。因此,寻找肝癌早期诊断方法,对肝癌患者的早期筛查、早期诊断及生存率提高有重大意义。

[1]
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019[J]. CA Cancer J Clin, 2019, 69(1):7-34.
[2]
Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3):209-249.
[3]
杨改标, 李倩, 李璇, 等. 血清AFP, B7-H4联合CP检测对原发性肝癌的诊断价值[J]. 大理大学学报, 2020, 5(10):75-79.
[4]
Zhao YJ, Ju Q, Li GC. Tumor markers for hepatocellular carcinoma[J]. Mol Clin Oncol, 2013, 1(4):593-598.
[5]
Waidely E, Al-Yuobi AR, Bashammakh AS, et al. Serum protein biomarkers relevant to hepatocellular carcinoma and their detection[J]. Analyst, 2016, 141(1):36-44.
[6]
谈艳芳, 刘利洪, 袁成良. AFP, AFP-L3/AFP, PIVKA-Ⅱ联合检测对原发性肝癌的诊断价值[J]. 检验医学与临床, 2021, 18(20): 2970-2972.
[7]
刘荣静, 冯培, 习浩. PIVKA-Ⅱ联合AFP, AFP-L3诊断原发性肝癌的Meta分析[J]. 检验医学与临床, 2020, 17(12):1648-1650.
[8]
Tayob N, Kanwal F, Alsarraj A, et al. The performance of AFP, AFP-3, DCP as biomarkers for detection of hepatocellular carcinoma (HCC): a phase 3 biomarker study in the United States[J]. Clin Gastroenterol Hepatol, 2023, 21(2):415-423, e4.
[9]
欧美同学会医师协会肝胆分会, 中国研究型医院学会分子诊断专委会, 中国临床肿瘤学会肝癌专委会, 等. 肝胆肿瘤分子诊断临床应用专家共识[J]. 中华肝胆外科杂志, 2020, 26(2):81-89.
[10]
Mao Y, Yang H, Xu H, et al. Golgi protein 73 (GOLPH2) is a valuable serum marker for hepatocellular carcinoma[J]. Gut, 2010, 59(12):1687-1693.
[11]
Jiao C, Cui L, Piao J, et al. Clinical significance and expression of serum Golgi protein 73 in primary hepatocellular carcinoma[J]. J Cancer Res Ther, 2018, 14(6):1239-1244.
[12]
Liu Y, Zhou S, Shi J, et al. c-Myc transactivates GP73 and promotes metastasis of hepatocellular carcinoma cells through GP73-mediated MMP-7 trafficking in a mildly hypoxic microenvironment[J]. Oncogenesis, 2019, 8(10):58.
[13]
Ye QH, Zhu WW, Zhang JB, et al. GOLM1 modulates EGFR/RTK cell-surface recycling to drive hepatocellular carcinoma metastasis[J]. Cancer Cell, 2016, 30(3):444-458.
[14]
Liu Y, Zhang X, Zhou S, et al. Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase-2 in hepatocellular carcinoma cells and inhibits cell invasion[J]. J Cell Mol Med, 2019, 23(4):2399-2409.
[15]
Bootcov MR, Bauskin AR, Valenzuela SM, et al. MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily[J]. Proc Natl Acad Sci USA, 1997, 94(21):11514-11519.
[16]
Liu X, Chi X, Gong Q, et al. association of serum level of growth differentiation factor 15 with liver cirrhosis and hepatocellular carcinoma[J]. Plos One, 2015, 10(5):e0127518.
[17]
Myojin Y, Hikita H, Sugiyama M, et al. Hepatic stellate cells in hepatocellular carcinoma promote tumor growth via growth differentiation factor 15 production[J]. Gastroenterology, 2021, 160(5):1741-1754, e16.
[18]
Kuang Z, Huang R, Yang Z, et al. Quantitative screening of serum protein biomarkers by reverse phase protein arrays[J]. Oncotarget, 2018, 9(66):32624-32641.
[19]
Wong N, Wang X. miRDB: an online resource for microRNA target prediction and functional annotations[J]. Nucleic Acids Res, 2015, 43(Database issue):D146-152.
[20]
李建华, 韩玲. 微小RNA-128对肝癌细胞增殖和凋亡的影响和机制[J]. 中华肝胆外科杂志, 2020, 26(1):57-60.
[21]
胡建军, 郑华荣, 郑耀红. miR-363下调HepG2细胞Mcl-1蛋白的表达并诱导其凋亡[J]. 中国病理生理杂志, 2015, 31(7):1329-1333.
[22]
Wei Y, Chen X, Liang C, et al. A noncoding regulatory RNAs network driven by circ-CDYL acts specifically in the early stages hepatocellular carcinoma[J]. Hepatology, 2020, 71(1):130-147.
[23]
Quinn JJ, Zhang QC, Georgiev P, et al. Rapid evolutionary turnover underlies conserved lncRNA-genome interactions[J]. Genes Dev, 2016, 30(2):191-207.
[24]
赵一鉴, 刘敏, 荆冠军. Lnc-MALAT1在肝癌组织中的表达及对化疗敏感性的影响[J]. 实用肿瘤杂志, 2020, 35(1):53-58.
[25]
Lao Y, Li Q, Li N, et al. Long noncoding RNA ENST00000455974 plays an oncogenic role through up-regulating JAG2 in human DNA mismatch repair-proficient colon cancer[J]. Biochem Biophys Res Commun, 2019, 508(2):339-347.
[26]
Li H, An J, Wu M, et al. LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2[J]. Oncotarget, 2015, 6(29):27847-27864.
[27]
Yu Y, Nangia-Makker P, Farhana L, et al. A novel mechanism of lncRNA and miRNA interaction:CCAT2 regulates miR-145 expression by suppressing its maturation process in colon cancer cells[J]. Mol Cancer, 2017, 16(1):155.
[28]
Leslie NR, Downes CP. PTEN function: how normal cells control it and tumour cells lose it[J]. Biochem J, 2004, 382(Pt 1):1-11.
[29]
Steinbichler TB, Dudás J, Riechelmann H, et al. The role of exosomes in cancer metastasis[J]. Semin Cancer Biol, 2017(44): 170-181.
[30]
Zhu C, Su Y, Liu L, et al. Circular RNA hsa_circ_0004277 stimulates malignant phenotype of hepatocellular carcinoma and epithelial-mesenchymal transition of peripheral cells[J]. Front Cell Dev Biol, 2021(8):585565.
[31]
潘韵芝, 马赛, 曹凯悦, 等. 高表达ANXA2肝癌干细胞外泌体对肝癌细胞恶性生物学特性的调控作用[J]. 中国肿瘤, 2019, 28(4):308-314.
[32]
汪晓华, 陈晓军. 肝癌患者外周血外泌体miR-25和miR-141表达水平及联合检测诊断效能研究[J]. 中国卫生检验杂志, 2020, 30(4):472-474.
[33]
Wang H, Hou L, Li A, et al. Expression of serum exosomal microRNA-21 in human hepatocellular carcinoma[J]. Biomed Res Int, 2014:864894.
[34]
Ye Q, Ling S, Zheng S, et al. Liquid biopsy in hepatocellular carcinoma: circulating tumor cells and circulating tumor DNA[J]. Mol Cancer, 2019, 18(1):114.
[35]
Pantel K, Alix-Panabières C. Functional studies on viable circulating tumor cells[J]. Clin Chem, 2016, 62(2):328-334.
[36]
Ahn JC, Teng PC, Chen PJ, et al. Detection of circulating tumor cells and their implications as a biomarker for diagnosis, prognostication, and therapeutic monitoring in hepatocellular carcinoma[J]. Hepatology, 2021, 73(1):422-436.
[37]
Qi LN, Xiang BD, Wu FX, et al. Circulating tumor cells undergoing EMT provide a metric for diagnosis and prognosis of patients with hepatocellular carcinoma[J]. Cancer Res, 2018, 78(16):4731-4744.
[38]
Xu W, Cao L, Chen L, et al. Isolation of circulating tumor cells in patients with hepatocellular carcinoma using a novel cell separation strategy[J]. Clin Cancer Res, 2011, 17(11):3783-3793.
[39]
Li J, Chen L, Zhang X, et al. Detection of circulating tumor cells in hepatocellular carcinoma using antibodies against asialoglycoprotein receptor, carbamoyl phosphate synthetase1and pan-cytokeratin[J]. PLoS One, 2014, 9(4):e96185.
[40]
Liu Z, Guo W, Zhang D, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry[J]. Sci Rep, 2016(6):39808.
[41]
Jiang P, Xie T, Ding SC, et al. Detection and characterization of jagged ends of double-stranded DNA in plasma[J]. Genome Res, 2020, 30(8):1144-1153.
[42]
Chen L, Abou-Alfa GK, Zheng B, et al. Genome-scale profiling of circulating cell-free DNA signatures for early detection of hepatocellular carcinoma in cirrhotic patients[J]. Cell Res, 2021, 31(5):589-592.
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