bFGF和PDGF在肝包虫囊壁表达及其与囊壁纤维化相关性分析

doi:10.3877/cma.j.issn.2095-3232.2026.02.016

目的

探究碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子（PDGF）在肝包虫囊壁及周围肝组织中表达及其与肝纤维化关系。

方法

标本来源于2020年6月至2024年8月新疆医科大学附属中医医院手术治疗的82例泡型肝包虫患者。患者均签署患者知情同意书，符合医学伦理学规定。其中男51例，女31例；年龄19~59岁，中位年龄38岁。收集患者术中切除的囊壁组织及病灶旁组织。所有组织部位均进行纤维化分级评分，通过实时荧光定量PCR及Western blot分别测定入组患者各组织样本中bFGF、PDGF-A、PDGF-B的mRNA水平及蛋白水平。不同组织中bFGF、PDGF-A、PDGF-B水平比较采用t检验，纤维化程度比较采用秩和检验，bFGF、PDGF-A、PDGF-B与肝纤维化程度相关性采用Spearman或Pearson相关性分析。

结果

囊壁组织Knodell纤维化1分7例、2分例、3分24例，病灶旁中Knodell纤维化0分72例、1分8例、2分2例，差异有统计学意义（Z=11.475，P<0.001）。囊壁组织bFGF、PDGF-A、PDGF-B mRNA相对表达量分别为2.27±0.56、1.74±0.43、1.61±0.33，病灶旁相应为0.61±0.13、0.41±0.12、0.56±0.11，差异有统计学意义（t=26.251，23.213，27.223；P<0.001）；两组蛋白相对表达量分别为2.11±0.59、1.94±0.48、1.52±0.34和0.55±0.14、0.44±0.12、0.49±0.12，组间差异亦有统计学意义（t=27.368，26.972，25.989；P<0.001）。相关性分析显示，囊壁组织bFGF、PDGF-A、PDGF-B mRNA相对表达量与囊壁纤维化程度呈正相关性（r_s=0.688，0.787，0.647；P<0.001）；囊壁组织bFGF、PDGF-A、PDGF-B mRNA相对表达量之间互相呈正相关性（r=0.673，0.604，0.704；P<0.001）。

结论

泡型肝包虫囊壁组织中bFGF、PDGF-A、PDGF-B水平明显高于周围肝组织，且与囊壁纤维化病变严重程度密切相关。

关键词: 肝包虫, 碱性成纤维细胞生长因子, 血小板衍生生长因子

Objective

To investigate the expression of basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) in hepatic hydatid cyst wall and surrounding liver tissues and their correlation with liver fibrosis.

Methods

Clinical specimens were collected from 82 patients with hepatic alveolar echinococcosis undergoing surgery in Traditional Chinese Medicine Hospital of Xinjiang Medical University from June 2020 to August 2024. The informed consents of all patients were obtained and the local ethical committee approval was received. Among them, 51 patients were male and 31 female, aged from 19 to 59 years, with a median age of 38 years. Intraoperatively, cyst wall tissues and the tissues adjacent to the lesions were collected. All tissues were subject to liver fibrosis staging and scoring. The expression profile of bFGF, PDGF-A and PDGF-B at mRNA and protein levels in all tissues were determined by real-time fluorescence quantitative PCR and Western blot, respectively. The expression levels of bFGF, PDGF-A and PDGF-B in different tissues were compared by t-test. The stage of liver fibrosis was compared by rank-sum test. The correlation between bFGF, PDGF-A and PDGF-B and the stage of liver fibrosis was assessed by Spearman or Pearson correlation analyses.

Results

Knodell fibrosis score of the cyst wall was 1 in 7 patients, 2 in 51 cases and 3 in 24 cases, respectively. Knodell fibrosis score of tissues adjacent to the lesions was 0 in 72 patients, 1 in 8 cases and 2 in 2 cases, respectively. The difference was statistically significant (Z=11.475, P<0.001). The relative expression levels of bFGF, PDGF-A and PDGF-B mRNA in the cyst wall were 2.27±0.56, 1.74±0.43 and 1.61±0.33, and 0.61±0.13, 0.41±0.12 and 0.56±0.11 in tissues adjacent to the lesions, respectively. The difference was statistically significant (t=26.251,23.213,27.223; all P<0.001). The relative expression levels of proteins in two groups were 2.11±0.59, 1.94±0.48, 1.52±0.34, and 0.55±0.14, 0.44±0.12, 0.49±0.12, respectively. The differences were also statistically significant (t=27.368, 26.972, 25.989; all P<0.001). Correlation analysis showed that the relative expression levels of bFGF, PDGF-A and PDGF-B mRNA in the cystic wall tissues were positively correlated with the stage of fibrosis (r_s=0.688, 0.787, 0.647; all P<0.001). A significant positive correlation was observed in the relative expression levels of bFGF, PDGF-A and PDGF-B mRNA (r=0.673, 0.604, 0.704; all P<0.001).

Conclusions

The expression levels of bFGF, PDGF-A and PDGF-B in the cyst wall of alveolar echinococcosis are significantly higher than those in the surrounding liver tissues, which are intimately correlated with the severity of fibrosis in the cyst wall.

Key words: Hepatic echinococcosis, Basic fibroblast growth factor, Platelet-derived growth factor

表1 肝包虫囊壁与病灶旁组织bFGF、PDGF-A、PDGF-B水平比较(

x ¯

±s)

组别	例数	bFGF		PDGF-A		PDGF-B
组别	例数	mRNA	蛋白	mRNA	蛋白	mRNA	蛋白
囊壁	82	2.27±0.56	2.11±0.59	1.74±0.43	1.94±0.48	1.61±0.33	1.52±0.34
病灶旁	82	0.61±0.13	0.55±0.14	0.41±0.12	0.44±0.12	0.56±0.11	0.49±0.12
t值		26.251	23.213	27.223	27.368	26.972	25.989
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

表1 肝包虫囊壁与病灶旁组织bFGF、PDGF-A、PDGF-B水平比较(

x ¯

±s)

组别	例数	bFGF		PDGF-A		PDGF-B
组别	例数	mRNA	蛋白	mRNA	蛋白	mRNA	蛋白
囊壁	82	2.27±0.56	2.11±0.59	1.74±0.43	1.94±0.48	1.61±0.33	1.52±0.34
病灶旁	82	0.61±0.13	0.55±0.14	0.41±0.12	0.44±0.12	0.56±0.11	0.49±0.12
t值		26.251	23.213	27.223	27.368	26.972	25.989
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

表2 不同纤维化程度的肝包虫囊壁组织间bFGF、PDGF-A、PDGF-B水平比较(

x ¯

±s)

Knodell纤维化评分	例数	bFGF		PDGF-A		PDGF-B
Knodell纤维化评分	例数	mRNA	蛋白	mRNA	蛋白	mRNA	蛋白
1分	7	0.99±0.28	1.23±0.17	1.07±0.25	1.23±0.37	1.01±0.17	1.10±0.13
2分	51	2.22±0.32	1.89±0.24	1.62±0.26	1.82±0.34	1.55±0.24	1.41±0.22
3分	24	2.75±0.35	2.85±0.43	2.21±0.29	2.42±0.33	1.89±0.26	1.87±0.29
F值		80.388	114.074	63.901	41.708	40.177	42.171
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

表2 不同纤维化程度的肝包虫囊壁组织间bFGF、PDGF-A、PDGF-B水平比较(

x ¯

±s)

Knodell纤维化评分	例数	bFGF		PDGF-A		PDGF-B
Knodell纤维化评分	例数	mRNA	蛋白	mRNA	蛋白	mRNA	蛋白
1分	7	0.99±0.28	1.23±0.17	1.07±0.25	1.23±0.37	1.01±0.17	1.10±0.13
2分	51	2.22±0.32	1.89±0.24	1.62±0.26	1.82±0.34	1.55±0.24	1.41±0.22
3分	24	2.75±0.35	2.85±0.43	2.21±0.29	2.42±0.33	1.89±0.26	1.87±0.29
F值		80.388	114.074	63.901	41.708	40.177	42.171
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

图1 不同纤维化程度的肝包虫囊壁组织间bFGF、PDGF-A、PDGF-B水平特征注：bFGF为碱性成纤维细胞生长因子，PDGF为血小板衍生生长因子；****为P<0.001

图2 囊壁纤维化程度与bFGF、PDGF-A、PDGF-B mRNA水平的相关性热图注：bFGF为碱性成纤维细胞生长因子，PDGF为血小板衍生生长因子

图3 肝包虫囊壁组织中bFGF、PDGF-A、PDGF-B mRNA线性关系注：bFGF为碱性成纤维细胞生长因子，PDGF为血小板衍生生长因子

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bFGF and PDGF expression profile in hepatic hydatid cyst wall and their correlation with cyst wall fibrosis

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bFGF and PDGF expression profile in hepatic hydatid cyst wall and their correlation with cyst wall fibrosis