Long noncoding RNA PTENP1 inhibits proliferation and migration of hepatocellular carcinoma cells

doi:10.3877/cma.j.issn.2095-3232.2016.02.013

Abstract

Abstract:

Objective

To investigate the effect and mechanism of long noncoding RNA (lncRNA) PTEN pseudogene 1 (PTENP1) on the proliferation and migration of hepatocellular carcinoma (HCC) cells.

Methods

Lentiviral vectors expressing PTENP1 were constructed. HCC cells BEL-7404 were infected with LV003-GFP-PTENP1 and control vectors LV003-GFP. BEL-7404 cells stably expressing PTENP1 were constructed and the experimental and control groups were established. The proliferation and clone formation abilities of HCC cells in two groups were detected by CCK-8 assay and clonogenic assay. The migration ability of HCC cells was detected by wound healing assay. The expression of p44/42 mitogen-activated protein kinase (MAPK) and p38 MAPK proteins were detected by Western blot.

Results

The absorbance values A₄₅₀ of the cells at 48 and 72 h in the experimental group were 1.4±0.3 and 2.3±1.1, significantly lower compared with 3.2±1.7 and 3.4±1.1 in the control group (t=-5.78,-4.23; P<0.05). The number of cell clone formation in the experimental group was 55±12, significantly less than 154±45 in the control group (t= -3.98, P<0.05). The percentage of cell migration in the experimental group was (21.7±2.6)%, significantly lower than (57.7±4.9)% in the control group (t=-8.34, P<0.05). Western blot revealed that the expression of p44/42 MAPK and p38 MAPK proteins in the experimental group was significantly down-regulated compared with those in the control group.

Conclusion

lncRNA PTENP1 can inhibit the proliferation and migration of HCC cells probably through regulating MAPK signaling pathway.

Key words: Ribonucleases, Long non-coding RNA, PTENP1, Liver neoplasms

Zhiyong Xiong, Zhicheng Yao, Weiming Fan, Mingliang Li, Kunpeng Hu, Jianliang Xu, Yuesi Zhong, Ruiyun Xu, Meihai Deng. Long noncoding RNA PTENP1 inhibits proliferation and migration of hepatocellular carcinoma cells[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2016, 05(02): 119-123.

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References 17

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