Mechanism of activated hepatic stellate cells promote angiogenesis of hepatocellular carcinoma

doi:10.3877/cma.j.issn.2095-3232.2016.05.013

Abstract

Abstract:

Objective

To investigate the mechanism of activated hepatic stellate cells (aHSC) promote the angiogenesis of hepatocellular carcinoma (HCC).

Methods

aHSCs were collected after being cultured for 1, 3, 5, 7 d respectively . The relative expression of aHSC angiopoietin-1 (Ang-1) messenger ribonucleic acid (mRNA) was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). The expression of aHSC Ang-1 protein was observed by immunofluorescence staining. The impact of aHSC on the proliferation of hepatic vascular endothelial cells (HVEC) was observed by Transwell chamber. The impact of aHSC on HVEC tube formation was observed by tube formation assay. The experimental data were compared using one-way analysis of variance and LSD-t test.

Results

After aHSC were cultured for 1, 3, 5, 7 d, the relative expression of aHSC Ang-1 mRNA was respectively 1.000±0.024, 1.920±0.080, 6.230±0.320 and 7.820±0.380, which gradually increased as the culture time went on (LSD-t=7.32, 13.68, 8.34; P<0.05). The immunofluorescence staining showed that Ang-1 and smooth muscle actin antibody (aSMA) were co-expressed in the aHSC. Transwell chamber indirect co-culture showed that aHSC could promote the proliferation of HVEC, and the effect weakened after Ang-1 antibody was added. Tube formation assay showed that HVEC could polymerize and gradually developed tubular structure in the aHSC conditioned medium, and the effect significantly weakened after Ang-1 antibody was added.

Conclusion

aHSC may promote the proliferation and angiogenesis of HVEC in HCC by secreting Ang-1.

Key words: Carcinoma, hepatocellular, Activited hepatic stellate cell, Angiopoietin-1, Angiogenesis

Yajun Tang, Chuzhi Pan, Yi Lu, Zhaofeng Tang, Nan Lin. Mechanism of activated hepatic stellate cells promote angiogenesis of hepatocellular carcinoma[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2016, 05(05): 323-327.

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