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中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2017, Vol. 06 ›› Issue (01) : 64 -68. doi: 10.3877/cma.j.issn.2095-3232.2017.01.014

所属专题： 文献；

基础研究

大范围肝组织淤血对大鼠肝切除术后早期残肝功能及肝再生能力的影响
李敬东1,†(), 闫在华2, 唐小龙2, 谢梦忆2, 冷政伟1   
  1. 1. 637007　四川省南充市，川北医学院附属医院肝胆外科；637007　四川省南充市，川北医学院肝胆胰肠研究所
    2. 637007　四川省南充市，川北医学院肝胆胰肠研究所
  • 收稿日期:2016-10-15 出版日期:2017-02-10
  • 通信作者: 李敬东

Effects of extensive liver congestion on the remnant liver function and liver regeneration in early stage after hepatectomy in rats

Jingdong Li1,†(), Zaihua Yan2, Xiaolong Tang2, Mengyi Xie2, Zhengwei Leng1   

  1. 1. Department of Hepatobiliary Surgery, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, China; Hepatobiliary, Pancreatic & Intestinal Diseases Research Institute, North Sichuan Medical College, Nanchong 637007, China
    2. Hepatobiliary, Pancreatic & Intestinal Diseases Research Institute, North Sichuan Medical College, Nanchong 637007, China
  • Received:2016-10-15 Published:2017-02-10
  • Corresponding author: Jingdong Li
  • About author:
    Corresponding author: Li Jingdong, Email:
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李敬东, 闫在华, 唐小龙, 谢梦忆, 冷政伟. 大范围肝组织淤血对大鼠肝切除术后早期残肝功能及肝再生能力的影响[J]. 中华肝脏外科手术学电子杂志, 2017, 06(01): 64-68.

Jingdong Li, Zaihua Yan, Xiaolong Tang, Mengyi Xie, Zhengwei Leng. Effects of extensive liver congestion on the remnant liver function and liver regeneration in early stage after hepatectomy in rats[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2017, 06(01): 64-68.

目的

探讨大范围肝组织淤血对大鼠肝切除术后早期残肝功能及肝再生能力的影响。

方法

健康雄性SD大鼠50只，按随机数字表法随机分为淤血组和非淤血组，每组25只。淤血组行肝中叶+左叶切除+尾状叶静脉结扎，非淤血组行肝中叶+左叶切除。检测术后24 h的ALT、AST、TB、血氨。Western blot检测肝组织cleaved caspase-3和增殖细胞核抗原（PCNA）蛋白表达。观察两组术后生存情况，检测指标比较采用t检验，生存分析采用Kaplan-Meier法和Log-rank检验。

结果

术后24 h，淤血组的ALT、AST、TB、血氨分别为（638±34）U/L、（675±26）U/L、（1.94±0.07）μmol/L、（250±23）μmol/L，明显高于非淤血组的（362±28）U/L、（412±20）U/L、（1.47±0.11）μmol/L、（131±19）μmol/L（t=14.09，17.88，8.05，8.82；P<0.05）；淤血组肝组织出现明显肝细胞空泡变性。淤血组肝组织cleaved caspase-3蛋白表达量为1.52±0.15，明显高于非淤血组的1.09±0.12，PCNA蛋白表达量为0.63±0.14，明显低于非淤血组的1.13±0.15（t=5.06，-5.55；P<0.05）。淤血组生存率为20%，明显低于非淤血组的75%（χ2=18.21，P<0.05）。

结论

大范围的肝组织淤血可促进大鼠肝切除术后肝组织细胞凋亡，降低肝再生能力，同时加重早期残肝功能的损害。

关键词: 大鼠, 模型，动物, 肝切除术, 肝功能试验, 肝再生
Objective

To investigate the effects of extensive liver congestion on the remnant liver function and liver regeneration in the early stage after hepatectomy in rats.

Methods

According to the random number table method, 50 healthy, male SD rats were randomly divided into the congestion and non-congestion groups with 25 rats in each group. In the congestion group, middle and left lobectomy of liver + caudate lobe venous ligation was performed. In the non-congestion group, middle and left lobectomy of liver was performed. The levels of ALT, AST, TB and plasma ammonia were detected at 24 h after operation. The expression levels of cleaved caspase-3 and proliferating cell nuclear antigen (PCNA) proteins in liver tissues were detected by Western blot. Postoperative survival situation of two groups was observed, the detection indexes were compared using t test, and the survival analysis was performed by Kaplan-Meier method and Log-rank test.

Results

At 24 h after operation, the levels of ALT, AST, TB and plasma ammonia in the congestion group was respectively (638±34) U/L, (675±26) U/L, (1.94±0.07) μmol/L and (250±23) μmol/L, significantly higher than (362±28) U/L, (412±20) U/L, (1.47±0.11) μmol/L and (131±19) μmol/L in the non-congestion group (t=14.09, 17.88, 8.05, 8.82; P<0.05). Evident vacuolar degeneration of liver cells was observed in the congestion group. The expression of cleaved caspase-3 protein in the liver tissue of the congestion group was 1.52±0.15, significantly higher than 1.09±0.12 of the non-congestion group (t=5.06, P<0.05). The expression of PCNA protein in the liver tissue of the congestion group was 0.63±0.14, significantly lower than 1.13±0.15 of the non-congestion group (t=-5.55, P<0.05). The survival rate in the congestion group was 20%, significantly lower than 75% in the non-congestion group (χ2=18.21, P<0.05).

Conclusion

Extensive liver congestion can promote liver cell apoptosis, reduce liver regeneration and aggravate the damage of remnant liver function in early stage after hepatectomy in rats.

Key words: Rat, Models, animal, Hepatectomy, Liver function tests, Liver regeneration
图1 两组大鼠肝切除术后24 h肝组织病理学变化（HE ×400）
图2 Western blot检测两组大鼠肝切除术后肝组织内cleaved caspase-3和PCNA蛋白的表达水平
图3 两组大鼠肝切除术后Kaplan-Meier生存曲线
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