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中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2020, Vol. 09 ›› Issue (01) : 83 -86. doi: 10.3877/cma.j.issn.2095-3232.2020.01.018

所属专题： 文献；

基础研究

骨髓间充质干细胞对肝星状细胞的接触调节作用
木斯他巴·买买提热依木1, 陈景耀2, 林楠3, 曹彦龙1, 吴建华1, 林洋1,†()   
  1. 1. 844000　新疆喀什地区第一人民医院普通外科
    2. 518107　深圳，中山大学附属第七医院消化医学中心
    3. 844000　新疆喀什地区第一人民医院普通外科；510630　广州，中山大学附属第三医院肝胆外科
  • 收稿日期:2019-09-12 出版日期:2020-02-10
  • 通信作者: 林洋
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2016D01C016)

Contact regulation of bone marrow mesenchymal stem cells on hepatic stellate cells

Maimaitireyimu Musitaba·1, Jingyao Chen2, Nan Lin3, Yanlong Cao1, Jianhua Wu1, Yang Lin1,†()   

  1. 1. Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China
    2. Digestive Medicine Center, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China
    3. Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China; Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2019-09-12 Published:2020-02-10
  • Corresponding author: Yang Lin
  • About author:
    Corresponding author: Lin Yang, Email:
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引用本文：

木斯他巴·买买提热依木, 陈景耀, 林楠, 曹彦龙, 吴建华, 林洋. 骨髓间充质干细胞对肝星状细胞的接触调节作用[J]. 中华肝脏外科手术学电子杂志, 2020, 09(01): 83-86.

Maimaitireyimu Musitaba·, Jingyao Chen, Nan Lin, Yanlong Cao, Jianhua Wu, Yang Lin. Contact regulation of bone marrow mesenchymal stem cells on hepatic stellate cells[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2020, 09(01): 83-86.

目的

探讨骨髓间充质干细胞（BMSC）对肝星状细胞（HSC）的直接接触调节作用。

方法

建立HSC及BMSC共培养系（共培养组），共培养72 h后通过流式细胞术分离细胞，采用Western blot分别检测共培养组HSC中Notch通路相关受体（Notch1、Notch2、Notch3及Notch4）及BMSC中Notch相关配体（Dll1，Dll3，Dll4，Jag1及Jag2）的表达情况，以单独培养HSC及BMSC为对照组。两组蛋白表达比较采用t检验。

结果

共培养组中Notch1表达量为1.85±0.34，明显高于对照组的0.98±0.25（t=4.235，P<0.05）；而Notch3表达为0.40±0.12，明显低于对照组的1.00±0.08（t=-7.971，P<0.05）；Notch相关配体Jag1表达为2.03±0.30，明显高于对照组的0.99±0.13（t=6.386，P<0.05）。

结论

BMSC可能通过Jag1结合Noch1以直接接触机制调控HSC。

关键词: 间质干细胞, 骨髓, 肝星状细胞, 共同培养技术
Objective

To investigate the direct contact regulation of bone marrow mesenchymal stem cells (BMSCs) upon hepatic stellate cells (HSCs).

Methods

The co-culture system of HSC and BMSC (co-culture group) was established. After 72-h co-culture, the cells were isolated by flow cytometry. The expression levels of related receptors of Notch signaling pathway (Notch1, Notch2, Notch3 and Notch4) in HSCs and Notch-related ligands (DII1, DII3, DII4, Jag1 and Jag2) in BMSCs from the co-culture group were detected by Western blot. HSCs and BMSCs which were cultured separately were taken as the control group. The expression levels of proteins were statistically compared between two groups using t test.

Results

In the co-culture group, the expression level of Notch1 was 1.85±0.34, significantly higher than 0.98±0.25 in the control group (t=4.235, P<0.05). However, the expression of Notch3 was 0.40±0.12, significantly lower than 1.00±0.08 in the control group (t=-7.971, P<0.05). The expression level of Notch-related ligand Jag1 was 2.03±0.30, significantly higher compared with 0.99±0.13 in the control group (t=6.386, P<0.05).

Conclusion

BMSCs may regulate HSCs through combining Jag1 with Noch1 in a direct contact way.

Key words: Mesenchymal stem cells, Bone marrow, Hepatic stellate cell, Coculture techniques
图1 免疫荧光化学法测定肝星状细胞内α-SMA表达
图2 Western blot检测Notch相关受体表达
图3 Western blot检测Notch相关配体表达
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