切换至 "中华医学电子期刊资源库"

中华肝脏外科手术学电子杂志 ›› 2024, Vol. 13 ›› Issue (03) : 384 -388. doi: 10.3877/cma.j.issn.2095-3232.2024.03.021

所属专题: 综述

综述

外泌体在肝细胞癌诊疗中的应用
仝心语1, 谭凯1, 白亮亮1, 杜锡林1,()   
  1. 1. 710038 西安,空军军医大学第二附属医院普通外科
  • 收稿日期:2024-02-24 出版日期:2024-06-10
  • 通信作者: 杜锡林
  • 基金资助:
    陕西省自然科学基础研究计划(2020JM-337)

Application of exosomes in diagnosis and treatment of hepatocellular carcinoma

Xinyu Tong1, Kai Tan1, Liangliang Bai1, Xilin Du1,()   

  1. 1. Department of General Surgery, the Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China
  • Received:2024-02-24 Published:2024-06-10
  • Corresponding author: Xilin Du
引用本文:

仝心语, 谭凯, 白亮亮, 杜锡林. 外泌体在肝细胞癌诊疗中的应用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(03): 384-388.

Xinyu Tong, Kai Tan, Liangliang Bai, Xilin Du. Application of exosomes in diagnosis and treatment of hepatocellular carcinoma[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2024, 13(03): 384-388.

外泌体是细胞外囊泡,内含蛋白质、脂类、核酸、microRNA、长链非编码RNA(lncRNA)和环状RNA(circRNA)等多种生物活性物质。外泌体可以通过影响血管内皮细胞功能促进肝癌血管的生成和肿瘤的转移,还可以通过调节各种免疫细胞的数量和功能,影响免疫因子的分泌,致使肿瘤细胞免疫逃逸发生。一些外泌体所包含的物质表达含量的变化与肝癌疾病过程有密切关系。外泌体是与肿瘤进展密切相关的生物标志物,在所有的体液中均可检测到外泌体,在临床有广阔的应用前景。因此,探索外泌体与HCC病情变化的联系,对于HCC患者的早诊、早治有重要意义。

Exosomes are extracellular vesicles, which contain multiple bioactive substances, such as protein, lipid, nucleic acid, microRNA, long-chain noncoding RNA (lncRNA) and circular RNA (circRNA), etc. Exosomes can promote the angiogenesis and metastasis of hepatocellular carcinoma (HCC) by affecting the function of vascular endothelial cells. They can also affect the secretion of immune cytokines by regulating the number and function of multiple immune cells, resulting in immune escape of tumor cells. The changes in the expression levels of substances contained in certain exosomes are closely associated with the process of HCC. Exosomes are biomarkers intimately associated with tumor progression. Exosomes can be detected in all body fluids, which have broad prospects in clinical application. Therefore, understanding the relationship between exosomes and the changes of the course of HCC is of significance for early diagnosis and treatment of HCC.

[1]
Wang H, Lu Z, Zhao X. Tumorigenesis, diagnosis, and therapeutic potential of exosomes in liver cancer[J]. J Hematol Oncol, 2019, 12(1):133.
[2]
谈艳芳,刘利洪,袁成良. AFP、AFP-L3/AFP、PIVKA-Ⅱ联合检测对原发性肝癌的诊断价值[J]. 检验医学与临床, 2021, 18(20):2970-2972.
[3]
李叶晟,杨宗国,陈晹, 等. 肝癌血清外泌体环状RNA表达谱差异及其临床意义[J]. 肝胆胰外科杂志, 2022, 34(5):283-287.
[4]
孟俊,王俊青,费晓春, 等. 血浆外泌体circRNA诊断HCC联合检测模型的建立与验证[J]. 检验医学, 2022, 37(1):1-10.
[5]
张娇弟,马梦婷,张倩, 等. 肝癌来源的外泌体中miRNA的作用[J]. 检验医学与临床, 2022, 19(19):2725-2728.
[6]
Li X, Li C, Zhang L, et al. The significance of exosomes in the development and treatment of hepatocellular carcinoma[J]. Mol Cancer, 2020, 19(1):1.
[7]
Abudoureyimu M, Zhou H, Zhi Y, et al. Recent progress in the emerging role of exosome in hepatocellular carcinoma[J]. Cell Prolif, 2019, 52(2):e12541.
[8]
Wu Q, Zhou L, Lv D, et al. Exosome-mediated communication in the tumor microenvironment contributes to hepatocellular carcinoma development and progression[J]. J Hematol Oncol, 2019, 12(1):53.
[9]
Wang F, Li L, Piontek K, et al. Exosome miR-335 as a novel therapeutic strategy in hepatocellular carcinoma[J]. Hepatology, 2018, 67(3):940-954.
[10]
Zhou Y, Ren H, Dai B, et al. Hepatocellular carcinoma-derived exosomal miRNA-21 contributes to tumor progression by converting hepatocyte stellate cells to cancer-associated fibroblasts[J]. J Exp Clin Cancer Res, 2018, 37(1):324.
[11]
Ye L, Zhang Q, Cheng Y, et al. Tumor-derived exosomal HMGB1 fosters hepatocellular carcinoma immune evasion by promoting TIM-1 + regulatory B cell expansion[J]. J Immunother Cancer, 2018, 6(1):145.
[12]
Zhang H, Deng T, Ge S, et al. Exosome circRNA secreted from adipocytes promotes the growth of hepatocellular carcinoma by targeting deubiquitination-related USP7[J]. Oncogene, 2019, 38(15):2844-2859.
[13]
Wang H, Hou L, Li A, et al. Expression of serum exosomal microRNA-21 in human hepatocellular carcinoma[J]. Biomed Res Int, 2014: 864894.
[14]
Suehiro T, Miyaaki H, Kanda Y, et al. Serum exosomal microRNA-122 and microRNA-21 as predictive biomarkers in transarterial chemoembolization-treated hepatocellular carcinoma patients[J]. Oncol Lett, 2018, 16(3):3267-3273.
[15]
Li J, Li Z, Zheng W, et al. LncRNA-ATB: an indispensable cancer-related long noncoding RNA[J]. Cell Prolif, 2017, 50(6):e12381.
[16]
Ferrín G, Ranchal I, Llamoza C, et al. Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE[J]. Liver Int, 2014, 34(3):438-446.
[17]
Li R, Wang Y, Zhang X, et al. Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis[J]. Mol Cancer, 2019, 18(1):18.
[18]
Lv LH, Wan YL, Lin Y, et al. Anticancer drugs cause release of exosomes with heat shock proteins from human hepatocellular carcinoma cells that elicit effective natural killer cell antitumor responses in vitro[J]. J Biol Chem, 2012, 287(19):15874-15885.
[19]
Chai N, Chang HE, Nicolas E, et al. Properties of subviral particles of hepatitis B virus[J]. J Virol, 2008, 82(16):7812-7817.
[20]
Masciopinto F, Giovani C, Campagnoli S, et al. Association of hepatitis C virus envelope proteins with exosomes[J]. Eur J Immunol, 2004, 34(10):2834-2842.
[21]
Shen J, Huang CK, Yu H, et al. The role of exosomes in hepatitis, liver cirrhosis and hepatocellular carcinoma[J]. J Cell Mol Med, 2017, 21(5):986-992.
[22]
Ghafouri-Fard S, Esmaeili M, Taheri M. H19 lncRNA: roles in tumorigenesis[J]. Biomed Pharmacother, 2020, 123: 109774.
[23]
蒋佳红,李游,邓知敏, 等. 外泌体在肝癌中的应用研究[J]. 医学信息, 2020, 33(24):28-30, 34.
[24]
Fang JH, Zhang ZJ, Shang LR, et al. Hepatoma cell-secreted exosomal microRNA-103 increases vascular permeability and promotes metastasis by targeting junction proteins[J]. Hepatology, 2018, 68(4):1459-1475.
[25]
孙文兵,邢艳珑,涂蓉, 等. 肝癌细胞外泌体诱导巨噬细胞M2极化后促进肝癌细胞迁移及侵袭[J]. 中国免疫学杂志, 2022, 38(12):1463-1468.
[26]
Cheng L, Liu J, Liu Q, et al. Exosomes from melatonin treated hepatocellularcarcinoma cells alter the immunosupression status through STAT3 pathway in macrophages[J]. Int J Biol Sci, 2017, 13(6):723-734.
[27]
Nakano T, Chen IH, Wang CC, et al. Circulating exosomal miR-92b: its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence[J]. Am J Transplant, 2019, 19(12):3250-3262.
[28]
He H, Cao L, Wang Z, et al. Sinomenine relieves airway remodeling by inhibiting epithelial-mesenchymal transition through downregulating TGF-β1 and Smad3 expression in vitro and in vivo[J]. Front Immunol, 2021, 12: 736479.
[29]
邓敬桓,卢国栋,周静, 等. 晚期转移性肝癌血清外泌体介导TGF-β通路对HepG2细胞上皮间质转化的影响[J]. 中华肿瘤防治杂志, 2023, 30(2):84-92.
[30]
徐文,郝向芳,李红, 等. 原发性肝癌患者血清诱导人肝癌细胞上皮-间叶间变[J]. 肿瘤, 2008, 28(5):372-377.
[31]
部楠. 血清肿瘤标志物联合检测在肝癌诊断中的应用价值[J].医疗装备, 2022, 35(12):33-35.
[32]
李思雯,黄文杰. 肝细胞癌生物标志物的研究进展:对于临床诊断及预后的意义[J]. 内科急危重症杂志, 2023, 29(1):6-10.
[33]
邹珍珍,王坤,何璐, 等. 异常凝血酶原和甲胎蛋白联合检测在原发性肝癌中的临床价值[J]. 检验医学与临床, 2019, 16(12):1771-1773.
[34]
屈振,法镇中,汤建军, 等. 肝癌患者血清外泌体内miR-665的表达及临床意义[J]. 肝胆胰外科杂志, 2018, 30(6):453-456.
[35]
Shi M, Jiang Y, Yang L, et al. Decreased levels of serum exosomal miR-638 predict poor prognosis in hepatocellular carcinoma[J].J Cell Biochem, 2018, 119(6):4711-4716.
[36]
Liu W, Hu J, Zhou K, et al. Serum exosomal miR-125b is a novel prognostic marker for hepatocellular carcinoma[J]. Onco Targets Ther, 2017, 10: 3843-3851.
[37]
Wang Y, Zhang C, Zhang P, et al. Serum exosomal microRNAs combined with alpha-fetoprotein as diagnostic markers of hepatocellular carcinoma[J]. Cancer Med, 2018, 7(5):1670-1679.
[38]
Cho HJ, Baek GO, Seo CW, et al. Exosomal microRNA-4661-5p-based serum panel as a potential diagnostic biomarker for early-stage hepatocellular carcinoma[J]. Cancer Med, 2020, 9(15):5459-5472.
[39]
汪晓华,陈晓军. 肝癌患者外周血外泌体mir-25和mir-141表达水平及联合检测诊断效能研究[J]. 中国卫生检验杂志, 2020, 30(4):472-474.
[40]
Wei Y, Chen X, Liang C, et al. A noncoding regulatory RNAs network driven by circ-CDYL acts specifically in the early stages hepatocellular carcinoma[J]. Hepatology, 2020, 71(1):130-147.
[41]
Lu Y, Duan Y, Xu Q, et al. Circulating exosome-derived bona fide long non-coding RNAs predicting the occurrence and metastasis of hepatocellular carcinoma[J]. J Cell Mol Med, 2020, 24(2):1311-1318.
[42]
Lee YR, Kim G, Tak WY, et al. Circulating exosomal noncoding RNAs as prognostic biomarkers in human hepatocellular carcinoma[J]. Int J Cancer, 2019, 144(6):1444-1452.
[43]
Yang L, Peng X, Li Y, et al. Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma[J]. Mol Cancer, 2019, 18(1):78.
[44]
姜绍文,谢青. 液体活检在肝细胞肝癌早期筛查中的作用及研究进展[J]. 临床内科杂志, 2022, 39(12):802-806.
[45]
Zhang X, Wang Z, Tang W, et al. Ultrasensitive and affordable assay for early detection of primary liver cancer using plasma cell-free DNA fragmentomics[J]. Hepatology, 2022, 76(2):317-329.
[46]
叶婷丹,雷学忠. 早期肝癌无创诊断之匙——循环游离DNA联合型液体活检[J]. 临床肝胆病杂志, 2022, 38(4):923-926.
[47]
Varghese J, Kedarisetty C, Venkataraman J, et al. Combination of TACE and sorafenib improves outcomes in BCLC stages B/C of hepatocellular carcinoma: a single centre experience[J]. Ann Hepatol, 2017, 16(2):247-254.
[48]
Liu W, Zheng L, Zhang R, et al. Circ-ZEB1 promotes PIK3CA expression by silencing miR-199a-3p and affects the proliferation and apoptosis of hepatocellular carcinoma[J]. Mol Cancer, 2022, 21(1):72.
[49]
Lou G, Chen L, Xia C, et al. MiR-199a-modified exosomes from adipose tissue-derived mesenchymal stem cells improve hepatocellular carcinoma chemosensitivity through mTOR pathway[J]. J Exp Clin Cancer Res, 2020, 39(1):4.
[50]
Fu X, Liu M, Qu S, et al. Exosomal microRNA-32-5p induces multidrug resistance in hepatocellular carcinoma via the PI3K/Akt pathway[J]. J Exp Clin Cancer Res, 2018, 37(1):52.
[51]
Hirao A, Sato Y, Tanaka H, et al. MiR-125b-5p is involved in sorafenib resistance through ataxin-1-mediated epithelial-mesenchymal transition in hepatocellular carcinoma[J]. Cancers, 2021, 13(19):4917.
[52]
Wang G, Zhao W, Wang H, et al. Exosomal miR-744 inhibits proliferation and sorafenib chemoresistance in hepatocellular carcinoma by targeting PAX2[J]. Med Sci Monit, 2019, 25: 7209-7217.
[53]
Wu B, Jiang Y, Zhu F, et al. Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703[J]. Oncol Lett, 2016, 11(4):2545-2551.
[54]
章菊,叶书来,张昌龙, 等. HepG2细胞外泌体冲击树突状细胞介导的抗肿瘤作用[J]. 癌变·畸变·突变, 2019, 31(1):29-34.
[55]
Qu Z, Wu J, Wu J, et al. Exosomes derived from HCC cells induce sorafenib resistance in hepatocellular carcinoma both in vivo and in vitro[J]. J Exp Clin Cancer Res, 2016, 35(1):159.
[56]
Takahashi K, Yan IK, Kogure T, et al. Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer[J]. FEBS Open Bio, 2014, 4: 458-467.
[57]
Wang D, Xing N, Yang T, et al. Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis[J]. Cancer Med, 2020, 9(19):7218-7230.
[1] 刘昌玲, 张金丽, 张志, 李孝建, 汤文彬, 胡逸萍, 陈宾, 谢晓娜. 负载人脂肪干细胞外泌体的甲基丙烯酰化明胶水凝胶对人皮肤成纤维细胞增殖和迁移的影响[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 517-525.
[2] 宋勤琴, 李双汝, 李林, 杜鹃, 刘继松. 间充质干细胞源性外泌体在改善病理性瘢痕中作用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 550-553.
[3] 李华志, 曹广, 刘殿刚, 张雅静. 不同入路下行肝切除术治疗原发性肝细胞癌的临床对比[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 52-55.
[4] 常小伟, 蔡瑜, 赵志勇, 张伟. 高强度聚焦超声消融术联合肝动脉化疗栓塞术治疗原发性肝细胞癌的效果及安全性分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 56-59.
[5] 屈翔宇, 张懿刚, 李浩令, 邱天, 谈燚. USP24及其共表达肿瘤代谢基因在肝细胞癌中的诊断和预后预测作用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 659-662.
[6] 公宇, 廖媛, 尚梅. 肝细胞癌TACE术后复发影响因素及预测模型建立[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 818-824.
[7] 李一帆, 朱帝文, 任伟新, 鲍应军, 顾俊鹏, 张海潇, 曹耿飞, 阿斯哈尔·哈斯木, 纪卫政. 血GP73水平在原发性肝癌TACE疗效评价中的作用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 825-830.
[8] 刘敏思, 李荣, 李媚. 基于GGT与Plt比值的模型在HBV相关肝细胞癌诊断中的作用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 831-835.
[9] 焦振东, 惠鹏, 金上博. 三维可视化结合ICG显像技术在腹腔镜肝切除术治疗复发性肝癌中的应用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 859-864.
[10] 陈晓鹏, 王佳妮, 练庆海, 杨九妹. 肝细胞癌VOPP1表达及其与预后的关系[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 876-882.
[11] 袁雨涵, 杨盛力. 体液和组织蛋白质组学分析在肝癌早期分子诊断中的研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 883-888.
[12] 吴警, 吐尔洪江·吐逊, 温浩. 肝切除术前肝功能评估新进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 889-893.
[13] 邓万玉, 陈富, 许磊波. 肝硬化与非肝硬化乙肝相关性肝癌患者术后无复发生存比较及其影响因素分析[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 670-674.
[14] 王向前, 李清峰, 陈磊, 丘文丹, 姚志成, 李熠, 吴荣焕. 姜黄素抑制肝细胞癌索拉非尼耐药作用及其调控机制[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(05): 729-735.
[15] 董晓斌, 张静, 苏莎莎, 莎比亚·沙吾提, 盛好. 溃疡性结肠炎患者相关环状RNA 差异表达谱分析及功能研究[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 499-509.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?