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中华肝脏外科手术学电子杂志 ›› 2020, Vol. 09 ›› Issue (02) : 191 -195. doi: 10.3877/cma.j.issn.2095-3232.2020.02.021

所属专题: 文献

基础研究

基于生物信息学数据库探讨STX6基因在肝细胞癌中的表达及临床意义
梁梓明1, 叶林森1, 唐晖1, 宋来恩1, 顾世杰1, 易述红1,()   
  1. 1. 510630 广州,中山大学附属第三医院肝移植中心
  • 收稿日期:2019-12-25 出版日期:2020-04-10
  • 通信作者: 易述红
  • 基金资助:
    广东省自然科学基金(2016A030313224); 中国博士后科学基金资助项目(2019TQ0369)

Analysis of expression and clinical significance of STX6 gene in hepatocellular carcinoma based on bioinformatics database

Ziming Liang1, Linsen Ye1, Hui Tang1, Laien Song1, Shijie Gu1, Shuhong Yi1,()   

  1. 1. Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2019-12-25 Published:2020-04-10
  • Corresponding author: Shuhong Yi
  • About author:
    Corresponding author: Yi Shuhong, Email:
引用本文:

梁梓明, 叶林森, 唐晖, 宋来恩, 顾世杰, 易述红. 基于生物信息学数据库探讨STX6基因在肝细胞癌中的表达及临床意义[J/OL]. 中华肝脏外科手术学电子杂志, 2020, 09(02): 191-195.

Ziming Liang, Linsen Ye, Hui Tang, Laien Song, Shijie Gu, Shuhong Yi. Analysis of expression and clinical significance of STX6 gene in hepatocellular carcinoma based on bioinformatics database[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2020, 09(02): 191-195.

目的

基于生物信息学数据库探讨突触融合蛋白6(STX6)在肝细胞癌(HCC)中的表达及临床意义。

方法

通过BioGPS数据库分析STX6基因在人体正常组织中的表达情况;使用Oncomine数据库检索STX6在常见肿瘤中的表达情况,并对HCC中STX6基因的表达情况进行Meta分析;随后在Kaplan-Meier Plotter数据库中分析STX6表达对HCC患者总生存率(OS)及无进展生存率(PFS)的影响;最后通过String数据库探索与STX6相互作用的蛋白。

结果

数据库分析发现,STX6基因在人体各个正常组织包括正常肝组织中均有表达,其中在全血、NK细胞、髓系细胞中含量相对较高。从2002年至今,发现涉及STX6表达差异具有统计学意义的研究共有16项,其中STX6表达增高13项,表达降低3项。8项基因芯片研究显示,HCC组织中STX6表达水平均高于正常肝组织(Fold change=1.510,1.094,1.103,1.124,1.716,1.844,1.269,2.484;P<0.05);对这8项研究进行Meta分析发现,STX6在HCC中表达上调(Median rank=569,P<0.05)。STX6高表达组患者的OS和PFS较STX6低表达组明显降低(HR=1.91,1.62;P<0.05)。蛋白相互作用网络分析发现,VAMP4、VAMP8、VAMP3、NAPA、VPS51、STX16、STX12、STX5、SNAP23、VTI1A等蛋白与STX6具有明显相互作用,大多数蛋白参与细胞内外及细胞器之间囊泡运输等生理功能。

结论

基于肿瘤基因数据库分析发现,STX6在HCC组织中高表达,STX6表达与HCC患者预后相关,高表达患者预后差。

Objective

To analyze the expression level and clinical significance of syntaxin 6 (STX6) in hepatocellular carcinoma (HCC) based on bioinformatics database.

Methods

The expression of STX6 gene in the healthy human tissues was analyzed by BioGPS database. The expression of STX6 in common tumors was assessed by Oncomine database. The expression profile of STX6 gene in HCC was determined by Meta-analysis. The effect of STX6 expression on the overall survival (OS) and progression-free survival (PFS) of HCC patients was evaluated in Kaplan-Meier plotter database. The proteins interacting with STX6 were identified in String database.

Results

STX6 gene was found expressed in all normal human tissues including liver tissues, with high expression levels in the whole blood, NK cells and myeloid cells. From 2002 till now, 16 studies involving statistical significances in the STX6 expression have been identified, including 13 studies of up-regulated STX6 expression and 3 of down-regulated STX6 expression. 8 gene chip studies demonstrated that the expression level of STX6 in HCC tissues was significantly higher than that in normal liver tissues (Fold change=1.510, 1.094, 1.103, 1.124, 1.716, 1.844, 1.269, 2.484; P<0.05). Meta-analysis of the 8 studies showed that STX6 expression was up-regulated in HCC tissues (Median rank=569, P<0.05). The OS and PFS in STX6 high expression group were significantly lower than those in STX6 low expression group (HR=1.91, 1.62; P<0.05). Analysis of protein interaction network revealed that proteins, such as VAMP4, VAMP8, VAMP3, NAPA, VPS51, STX16, STX12, STX5, SNAP23 and VTI1A, significantly interacted with STX6. A majority of proteins participated in physiological functions such as vesicle transportation in and out of cells or between organelles.

Conclusions

The findings based on the analysis of tumor gene database demonstrate that STX6 is highly expressed in HCC tissue, STX6 expression is correlated with the prognosis of HCC patients, and the prognosis of patients with high expression of STX6 is poor.

图1 Oncomine数据库中STX6在不同HCC研究芯片中的表达
图2 STX6高表达组和低表达组HCC患者Kaplan-Meier生存曲线
图3 STX6基因表达蛋白相关的蛋白网络图
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