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中华肝脏外科手术学电子杂志 ›› 2021, Vol. 10 ›› Issue (02) : 206 -209. doi: 10.3877/cma.j.issn.2095-3232.2021.02.018

所属专题: 文献

基础研究

犬纤维蛋白胶粘结式胰肠吻合安全性研究
郝杰1, 雷鹏2, 张斌斌3, 吕毅1, 崔晓海4,()   
  1. 1. 710061 西安交通大学第一附属医院肝胆外科;710061 西安,精准外科与再生医学国家地方联合工程研究中心
    2. 750000 银川,宁夏医科大学总医院肝胆外科
    3. 030000 太原,山西大医院肝胆外科
    4. 710061 西安,精准外科与再生医学国家地方联合工程研究中心;710061 西安交通大学第一附属医院胸外科
  • 收稿日期:2021-01-06 出版日期:2021-04-10
  • 通信作者: 崔晓海
  • 基金资助:
    陕西省重点研发计划(2018SF-022); 陕西省自然科学基础研究计划(2020JM-402)

Study on safety of conglutinant pancreaticojejunostomy with fibrin glue in dog models

Jie Hao1, Peng Lei2, Binbin Zhang3, Yi Lyu1, Xiaohai Cui4,()   

  1. 1. Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China; National and Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, Xi'an 710061, China
    2. Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan 750000, China
    3. Department of Hepatobiliary Surgery, Shanxi Bethune Hospital, Taiyuan 030000, China
    4. National and Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, Xi'an 710061, China; Department of Thoracic Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China
  • Received:2021-01-06 Published:2021-04-10
  • Corresponding author: Xiaohai Cui
引用本文:

郝杰, 雷鹏, 张斌斌, 吕毅, 崔晓海. 犬纤维蛋白胶粘结式胰肠吻合安全性研究[J/OL]. 中华肝脏外科手术学电子杂志, 2021, 10(02): 206-209.

Jie Hao, Peng Lei, Binbin Zhang, Yi Lyu, Xiaohai Cui. Study on safety of conglutinant pancreaticojejunostomy with fibrin glue in dog models[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2021, 10(02): 206-209.

目的

探讨纤维蛋白胶粘结式胰肠吻合的可行性及安全性。

方法

选取10只家犬,于胰尾切除术后,采用纤维蛋白胶行粘结式胰肠吻合,分别于术前及术后1、3、7、10 d检测血清淀粉酶变化,并于术后10、30 d腹腔探查,取吻合口标本行病理学检查。

结果

粘结式胰肠吻合平均手术时间为(9.0±2.1)min。术前血清淀粉酶为(598±97)IU/L,术后10 d为(622±79)IU/L,术后无胰瘘发生。肉眼观察可见吻合口周围大网膜包裹,与周围组织广泛粘连,无胰瘘等形成;HE染色显示胰肠吻合口愈合良好,少量炎症细胞浸润。

结论

纤维蛋白胶用于犬粘结式胰肠吻合方法简单、操作简便、安全可靠,可有效缩短手术时间。

Objective

To explore the feasibility and safety of conglutinant pancreaticojejunostomy using fibrin glue.

Methods

Conglutinant pancreaticojejunostomy using fibrin glue was performed after pancreatectomy in 10 dog models. The changes of serum amylase level were detected before and at 1-, 3-, 7- and 10 d after operation. Abdominal cavity exploration was conducted at postoperative 10- and 30 d. The anastomotic specimens were collected for pathological examination.

Results

The average operation time of conglutinant pancreaticojejunostomy was (9.0±2.1) min. The serum amylase level was (598±97) IU/L before operation and (622±79) IU/L at 10 d after operation. No pancreatic fistula occurred after operation. Macroscopic observation found that the anastomotic site was surrounded by omentum, which was widely adhered to the surrounding tissues. No pancreatic fistula and other complications were observed. HE staining demonstrated that the anastomotic site of pancreaticojejunostomy healed well and slight inflammatory cell infiltration was noted.

Conclusions

It is convenient, safe and reliable to apply fibrin glue in conglutinant pancreaticojejunostomy in dog models, which can effectively shorten the operation time.

图1 犬胰肠吻合手术图
图2 犬胰肠吻合口术后情况
图3 胰肠吻合口组织切片情况
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