To investigate the protective effect and mechanism of naringin on oxidative damage of L02 liver cells.

Blank group (L02 liver cells only), naringin group (L02 liver cells with naringin 25 μmol/L), H₂O₂ damage group (damage group, L02 liver cells with H₂O₂ 200 μmol/L) and protection group (L02 liver cells with H₂O₂ 200 μmol/L and naringin 25 μmol/L) were assigned. Cell viability was evaluated by detecting cell absorbance value (A₄₈₈). Cell apoptosis rate was detected by flow cytometry. Mitochondrial membrane potential (ΔΨm) was detected by using 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetrethyl benzimidalyl carbocyanine iodide (JC-1). Malondialdehyde (MDA) was evaluated by detecting cell absorbance value (A₅₃₂). Reactive oxygen species (ROS) was evaluated by detecting fluorescence intensity by flow cytometry. Superoxide dismutase (SOD) specific activity was detected by using improved xanthine oxidase detection method. Data of these groups were compared by one-way analysis of variance and LSD-t test.

The average cell viability (A₄₈₈), cell apoptosis rate,ΔΨm were 0.702±0.010, (25.0±0.9)%, 0.95±0.03 respectively in damage group, 0.839±0.032, (14.8±1.7)%, 1.28±0.07 respectively in protection group, and 0.992±0.013, (6.2±0.5)%,1.71±0.08 respectively in blank group. There were significant differences in cell viability, cell apoptosis rate,ΔΨm between damage group and blank group (LSD-t= -27.44, 34.98, -19.88; P<0.05). There were significant differences in cell viability, cell apoptosis rate, Ψm between damage group and protection group (LSD-t=12.94, -18.91, 8.66; P<0.05). The MDA (A₅₃₂) , ROS (fluorescence intensity) and SOD specific activity were 0.610±0.051, 1 772±69, (67±2)U/g respectively in damage group, 0.346±0.008, 588±24, (111±6)U/g respectively in protective group, 0.121±0.003, 337±17, (159±5)U/g respectively in naringin group, and 0.123±0.007, 375±16, (142±5)U/g respectively in blank group. There were significant differences in ROS and SOD specific activity between naringin group and blank group (LSD-t= -2.24, 5.55; P<0.05). There were significant differences in MDA, ROS and SOD specific activity between damage group and blank group (LSD-t=31.66, 77.85, -24.12; P<0.05). There were significant differences in MDA, ROS and SOD specific activity between protective group and damage group (LSD-t= -17.12, -65.97, 14.03; P<0.05).

Naringin can protect L02 liver cell against oxidative damage by improving its antioxidant capacity.