切换至 "中华医学电子期刊资源库"

中华肝脏外科手术学电子杂志 ›› 2016, Vol. 05 ›› Issue (03) : 190 -194. doi: 10.3877/cma.j.issn.2095-3232.2016.03.015

所属专题: 文献

基础研究

小鼠肝切除术后肝种植瘤模型的建立
张俊斌1, 汪根树1,(), 周静2, 冯智英2, 刘慧玲3, 谌小龙1, 李慧1, 王励1, 李势辉1, 杨建旭1   
  1. 1. 510630 广州,中山大学附属第三医院器官移植中心
    2. 510630 广州,中山大学附属第三医院病理科
    3. 510630 广州,中山大学附属第三医院消化内科
  • 收稿日期:2016-02-17 出版日期:2016-06-10
  • 通信作者: 汪根树
  • 基金资助:
    广东省科技计划项目(2012B060300012); 广州市科技计划项目(2014J4100128)

Construction of mouse model of liver implanted tumor after hepatectomy

Junbin Zhang1, Genshu Wang1,(), Jing Zhou2, Zhiying Feng2, Huiling Liu3, Xiaolong Chen1, Hui Li1, Li Wang1, Shihui Li1, Jianxu Yang1   

  1. 1. Organ Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    2. Department of Pathology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    3. Department of Gastroenterology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2016-02-17 Published:2016-06-10
  • Corresponding author: Genshu Wang
  • About author:
    Corresponding author: Wang Genshu, Email:
引用本文:

张俊斌, 汪根树, 周静, 冯智英, 刘慧玲, 谌小龙, 李慧, 王励, 李势辉, 杨建旭. 小鼠肝切除术后肝种植瘤模型的建立[J/OL]. 中华肝脏外科手术学电子杂志, 2016, 05(03): 190-194.

Junbin Zhang, Genshu Wang, Jing Zhou, Zhiying Feng, Huiling Liu, Xiaolong Chen, Hui Li, Li Wang, Shihui Li, Jianxu Yang. Construction of mouse model of liver implanted tumor after hepatectomy[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2016, 05(03): 190-194.

目的

探讨肝癌组织块种植法小鼠肝切除术后肝种植瘤模型的建立。

方法

小鼠H22肝癌细胞接种至2只BALB/c(nu/nu)裸鼠肩胛部,2周后成瘤。按照随机数字表法将18只裸鼠分为无肝切除组(A组),30%肝切除组(B组)和70%肝切除组(C组),每组6只。建立裸鼠肝切除模型,A组裸鼠游离肝左外叶及中叶,B组结扎切除肝左外叶组织,C组结扎切除肝左外叶及中叶组织。取肩胛部肿瘤组织接种至3组裸鼠肝脏。比较3组成瘤率和种植瘤体积。成瘤率比较采用Fisher确切概率法。3组种植瘤体积比较采用Kruskal-Wallis秩和检验及Bonferroni法。

结果

2周后A、B、C组的成瘤率分别为6/6、6/6、5/6,差异无统计学意义(P=1.000);A、B、C组的种植瘤体积中位数分别为19(6~33)、22(8~44)和150(58~186)mm3,C组明显大于A组和B组(Z=9.03,7.12;P<0.0167)。组织病理学检查证实3组种植瘤均为肝细胞癌,伴有不同程度的坏死和炎症细胞浸润。

结论

肝癌组织块种植法可以建立理想的小鼠肝大部分切除术后肝种植瘤模型,具有实验周期短,成瘤率高以及稳定性好等优点。

Objective

To explore the construction of mouse model of liver implanted tumor after hepatectomy by implantation of liver tumor tissue.

Methods

H22 hepatocellular carcinoma (HCC) cells of mice were implanted to the scapula of 2 BALB/c (nu/nu) nude mice and tumors developed in 2 weeks. Eighteen nude mice were divided into three groups according to random number table method: non-hepatectomy group (group A), 30% hepatectomy group (group B) and 70% hepatectomy group (group C) with 6 mice in each group. The hepatectomy model of nude mice was established. The left lateral lobe and middle lobe were dissected for the nude mice in group A. The tissues of left lateral lobe were ligated and resected in group B. And the tissues of left lateralobe and middle lobe were ligated and resected in group C. The tumor tissues incised from the scapula were implanted to the livers of nude mice in three groups. The tumor formation rate and the volume of implanted tumors of three groups were compared. The tumor formation rate was compared using Fisher's exact probability test, and the volume of implanted tumors was compared using Kruskal-Wallis test and Bonferroni method.

Results

The tumor formation rate of group A, B and C after 2 weeks was respectively 6/6, 6/6 and 5/6, and no significant difference was observed (P=1.000). The median volume of implanted tumors in group A, B and C was respectively 19(6-33), 22(8-44) and 150(58-186) mm3. The median volume of implanted tumors in group C was significantly larger than those in group A and B (Z=9.03, 7.12; P<0.0167). The implanted tumors in three groups were all confirmed as HCC and with different degrees of necrosis and inflammatory cell infiltration by histopathological examination.

Conclusions

Implantation of liver tumor tissue can be used to construct an ideal mouse model of liver implanted tumor after major hepatectomy with the advantages of short experimental period, high tumor formation rate and good stability.

图1 裸鼠肩胛部皮下肝癌细胞种植瘤
图2 三组裸鼠原位种植瘤病理学检查
[1]
Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2): 87-108.
[2]
张琪,陈规划.肝癌肝移植——进展与展望[J].器官移植,2014,5(3): 141-144,173.
[3]
Mazzaferro V, Llovet JM, Miceli R, et al. Predicting survival after liver transplantation in patients with hepatocellular carcinoma beyond the Milan criteria: a retrospective, exploratory analysis[J]. Lancet Oncol, 2009, 10(1): 35-43.
[4]
Lee SY, Konstantinidis IT, Eaton AA, et al. Predicting recurrence patterns after resection of hepatocellular cancer[J]. HPB, 2014,16(10): 943-953.
[5]
Weng LL, Xiang JF, Lin JB, et al. Asparagus polysaccharide and gum with hepatic artery embolization induces tumor growth and inhibits angiogenesis in an orthotopic hepatocellular carcinoma model[J]. Asian Pac J Cancer Prev, 2014, 15(24): 10949-10955.
[6]
Wang J, Dong L, Xu L, et al. B cell CLL/lymphoma 6 member B inhibits hepatocellular carcinoma metastases in vitro and in mice[J]. Cancer Lett, 2014, 355(2): 192-200.
[7]
陈之巨,林楠,潘楚芝,等.活化态肝星状细胞对小鼠肝细胞肝癌血管形成、肿瘤生长及转移的作用[J/CD].中华肝脏外科手术学电子杂志,2013,2(3): 186-193.
[8]
Stöcker E, Pfeifer U. On the manner of proliferation of the liver parenchyma after partial hepatectomy. Autoradiography studies using 3H-thymidine[J]. Naturwissenschaften, 1965, 52(24): 663.
[9]
Raskopf E, Dzienisowicz C, Hilbert T, et al. Effective angiostatic treatment in a murine metastatic and orthotopic hepatoma model[J]. Hepatology, 2005, 41(6): 1233-1240.
[10]
Rupertus K, Sinistra J, Scheuer C, et al. Interaction of the chemokines I-TAC (CXCL11) and SDF-1 (CXCL12) in the regulation of tumor angiogenesis of colorectal cancer[J]. Clin Exp Metastasis, 2014, 31(4): 447-459.
[11]
Pancoska P, Carr BI. Macro- and micro-environmental factors in clinical hepatocellular cancer[J]. Semin Oncol, 2014, 41(2):185-194.
[12]
Ochoa B, Syn WK, Delgado I, et al. Hedgehog signaling is critical for normal liver regeneration after partial hepatectomy in mice[J]. Hepatology, 2010, 51(5): 1712-1723.
[13]
Grzelak CA, Martelotto LG, Sigglekow ND, et al. The intrahepatic signalling niche of hedgehog is defined by primary cilia positive cells during chronic liver injury[J]. J Hepatol, 2014, 60(1): 143-151.
[14]
金海,刘慧玲,李学俊,等.大鼠部分肝切除术后Hedgehog信号分子的表达[J].中国病理生理杂志,2012,28(1): 90-93.
[15]
Paschos KA, Bird NC. Liver regeneration and its impact on post-hepatectomy metastatic tumour recurrence[J]. Anticancer Res, 2010, 30(6): 2161-2170.
[16]
Frantsiiants EM, Kaplieva IV, Trepitaki LK, et al. Dynamics of growth factors in the spleen and liver of rats at different stages of metastatic hepatic reconstitution[J]. Eksp Klin Gastroenterol, 2014(10): 38-43.
[17]
Dragani TA, Manenti G, Gariboldi M, et al. Genetics of liver tumor susceptibility in mice[J]. Toxicol Lett, 1995(82/83): 613-619.
[18]
Demirci S, Doğan A, BaSak N, et al. A Schiff base derivative for effective treatment of diethylnitrosamine-induced liver cancer in vivo[J]. Anticancer Drugs, 2015, 26(5): 555-564.
[19]
Schneider C, Teufel A, Yevsa T, et al. Adaptive immunity suppresses formation and progression of diethylnitrosamine-induced liver cancer[J]. Gut, 2012, 61(12): 1733-1743.
[20]
Lu L, Han H, Tian Y, et al. Aurora kinase A mediates c-Myc's oncogenic effects in hepatocellular carcinoma[J]. Mol Carcinog, 2015, 54(11): 1467-1479.
[21]
郑建明,陶文照,郑唯强,等.人肝癌裸鼠皮下-肝原位移植瘤模型的建立[J].第二军医大学学报,2000,21(5): 456-459,502.
[22]
Shi JH, Huitfeldt HS, Suo ZH, et al. Growth of hepatocellular carcinoma in the regenerating liver[J]. Liver Transpl, 2011, 17(7): 866-874.
[1] 钟锴, 蒋铁民, 张瑞青, 吐尔干艾力·阿吉, 邵英梅, 郭强. 加速康复外科在肝囊型棘球蚴病肝切除术中的应用分析[J/OL]. 中华普通外科学文献(电子版), 2024, 18(06): 425-429.
[2] 刘世君, 马杰, 师鲁静. 胃癌完整系膜切除术+标准D2根治术治疗进展期胃癌的近中期随访研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 27-30.
[3] 李华志, 曹广, 刘殿刚, 张雅静. 不同入路下行肝切除术治疗原发性肝细胞癌的临床对比[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 52-55.
[4] 常小伟, 蔡瑜, 赵志勇, 张伟. 高强度聚焦超声消融术联合肝动脉化疗栓塞术治疗原发性肝细胞癌的效果及安全性分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 56-59.
[5] 冯旺, 马振中, 汤林花. CT扫描三维重建在肝内胆管细胞癌腹腔镜肝切除术中的临床研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 104-107.
[6] 赖全友, 高远, 汪建林, 屈士斌, 魏丹, 彭伟. 三维重建技术结合腹腔镜精准肝切除术对肝癌患者术后CD4+、CD8+及免疫球蛋白水平的影响[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 651-654.
[7] 唐梅, 周丽, 牛岑月, 周小童, 王倩. ICG荧光导航的腹腔镜肝切除术临床意义[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 655-658.
[8] 屈翔宇, 张懿刚, 李浩令, 邱天, 谈燚. USP24及其共表达肿瘤代谢基因在肝细胞癌中的诊断和预后预测作用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 659-662.
[9] 公宇, 廖媛, 尚梅. 肝细胞癌TACE术后复发影响因素及预测模型建立[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 818-824.
[10] 李一帆, 朱帝文, 任伟新, 鲍应军, 顾俊鹏, 张海潇, 曹耿飞, 阿斯哈尔·哈斯木, 纪卫政. 血GP73水平在原发性肝癌TACE疗效评价中的作用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 825-830.
[11] 刘敏思, 李荣, 李媚. 基于GGT与Plt比值的模型在HBV相关肝细胞癌诊断中的作用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 831-835.
[12] 焦振东, 惠鹏, 金上博. 三维可视化结合ICG显像技术在腹腔镜肝切除术治疗复发性肝癌中的应用[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 859-864.
[13] 陈晓鹏, 王佳妮, 练庆海, 杨九妹. 肝细胞癌VOPP1表达及其与预后的关系[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 876-882.
[14] 袁雨涵, 杨盛力. 体液和组织蛋白质组学分析在肝癌早期分子诊断中的研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 883-888.
[15] 吴警, 吐尔洪江·吐逊, 温浩. 肝切除术前肝功能评估新进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 889-893.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?