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中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2021, Vol. 10 ›› Issue (04) : 423 -427. doi: 10.3877/cma.j.issn.2095-3232.2021.04.018

基础研究

大鼠减体积自体肝移植模型的建立
李涛1, 沙地克·阿帕尔1, 吴警1, 张皓1, 江多斯·帕依孜吾拉1, 曾齐1, 曾齐努尔扎提江·安维尔1, 吐尔洪江·吐逊1,†()   
  1. 1. 830054　乌鲁木齐，新疆医科大学第一附属医院消化血管外科中心肝脏·腹腔镜外科
  • 收稿日期:2021-05-06 出版日期:2021-08-18
  • 通信作者: 吐尔洪江·吐逊
  • 基金资助:
    国家自然科学基金(81560329); 新疆维吾尔自治区科技厅重点实验室开放课题项目(2018D04002); 新疆维吾尔自治区天山青年计划博士科技人才培养项目(2017Q094)

Establishment of rat models of reduced-size autologous liver transplantation

Tao Li1, Apaer Shadike·1, Jing Wu1, Hao Zhang1, Payiziwula Jiangduosi·1, Qi Zeng1, Anweier Nuerzhatijiang·1, Tuxun Tuerhongjiang·1,†()   

  1. 1. Department of Liver and Laparoscopic Surgery, Digestive Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China
  • Received:2021-05-06 Published:2021-08-18
  • Corresponding author: Tuxun Tuerhongjiang·
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李涛, 沙地克·阿帕尔, 吴警, 张皓, 江多斯·帕依孜吾拉, 曾齐, 曾齐努尔扎提江·安维尔, 吐尔洪江·吐逊. 大鼠减体积自体肝移植模型的建立[J]. 中华肝脏外科手术学电子杂志, 2021, 10(04): 423-427.

Tao Li, Apaer Shadike·, Jing Wu, Hao Zhang, Payiziwula Jiangduosi·, Qi Zeng, Anweier Nuerzhatijiang·, Tuxun Tuerhongjiang·. Establishment of rat models of reduced-size autologous liver transplantation[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2021, 10(04): 423-427.

目的

探讨大鼠减体积自体肝移植模型的建立。

方法

20例SD大鼠在异氟烷吸入麻醉后，经门静脉肝脏原位冷灌注含有肝素钠（50 IU/ml）的乳酸钠林格液并切除肝中叶及左外叶，模拟临床自体肝移植手术过程，建立大鼠减体积自体肝移植模型。观察大鼠围手术期相关指标及存活情况。

结果

大鼠减体积自体肝移植模型20例，成功完成手术18例，1例术中死于分离肝上下腔静脉时损伤膈肌造成气胸，1例死于术后复苏体温过低而无法苏醒。大鼠平均麻醉诱导时间（1.9±0.3）min，手术时间（38.6±2.9）min，无肝期时间（16.0±0.9）min，切除肝重量（6.4±0.8）g，术后麻醉复苏时间（3.1±0.3）min。大鼠术后1周成活率94%（17/18）。

结论

大鼠减体积自体肝移植模型建立的操作相对简易，可重复性好，术后存活率高。该模型有效结合肝切除和原位肝移植技术，适合缺血-再灌注损伤和自体肝移植等机制研究。

关键词: 肝移植, 移植, 自体, 模型, 动物, 大鼠
Objective

To investigate the establishment of rat models of reduced-size autologous liver transplantation.

Methods

After isoflurane inhalation anesthesia, 20 SD rats were treated with cold perfusion in situ of sodium lactate Ringer's solution containing heparin sodium (50 IU/ml) via the portal vein, and then the middle and left lateral liver lobes were resected to simulate the clinical process of autologous liver transplantation. Relevant parameters and survival of rats during the perioperative period were observed.

Results

Among the 20 rats receiving reduced-size autologous liver transplantation, 18 successfully survived.1 rat died of pneumothorax caused by diaphragm injury when separating the superior and inferior vena cava of the liver intraoperatively, and 1 rat died of hypothermia after postoperative resuscitation. The mean anesthesia induction time, operation time, anhepatic time, resected liver mass and postoperative recovery time from anesthesia were (1.9±0.3) min, (38.6±2.9) min, (16.0±0.9) min, (6.4±0.8) g and (3.1±0.3) min, respectively. The survival rate of rats at postoperative 1 week was 94%(17/18).

Conclusions

It is relatively convenient and reproducible to establish the rat models of reduced-size autologous liver transplantation, which yields high postoperative survival rate. This rat model effectively combines liver resection and orthotopic liver transplantation, which is suitable for the mechanism study of ischemia-reperfusion injury and autologous transplantation.

Key words: Liver transplantation, Transplantation, autologous, Models, animal, Rats
图1 SD大鼠减体积自体肝移植模型建立过程
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