探讨荧光素酶标记肝细胞癌（肝癌）细胞的荧光成像技术在活体示踪裸鼠肝癌肺转移中的价值。

应用低转移潜能HCC97L、高转移潜能HCC97H肝癌细胞建立稳定表达荧光素酶的肝癌细胞株HCC97L-FL、HCC97H-FL。24只裸鼠按随机数字表法随机分为HCC97L-FL组和HCC97H-FL组。分别将5×10⁶个HCC97L-FL和HCC97H-FL细胞加入磷酸盐缓冲液（PBS）50 μl，制成细胞悬液。将细胞悬液注入裸鼠尾静脉，建立荧光素示踪的裸鼠肝癌肺转移模型。观察荧光成像示踪肺转移瘤大体标本及组织标本情况。两组数据比较采用t检验。

荧光检测发现，HCC97L-FL、HCC97H-FL细胞均可发出强烈荧光。建模后2～3周，HCC97H-FL组裸鼠的肺部收集到荧光信号，3～4周荧光强度逐渐增强；HCC97L-FL组裸鼠无可见的肺部荧光信号。建模后4周HCC97H-FL组荧光强度为（1.73 ± 0.16）×10⁴ a.u.，明显高于HCC97L-FL组的（0.03 ±0.01）×10⁴ a.u.（t=23.652, P<0.05）。建模后4周HCC97H-FL组裸鼠肺脏密布大小不等转移瘤病灶，部分较大病灶突出于肺脏表面；HCC97L-FL组中未观察到转移瘤。转移瘤癌细胞核异型性明显，具备肝癌特征。

荧光素酶标记肝癌细胞的荧光成像技术可对裸鼠肝癌肺转移进行活体、动态示踪。该技术为体内肝癌细胞生长、侵袭、转移的研究提供了可靠的量化研究手段。

To investigate the value of fluorescence imaging of labeling the hepatocellular carcinoma (HCC) cells with luciferase in tracking the pulmonary metastasis of HCC of nude mouse in vivo.

Low metastatic potential HCC97L and high metastatic potential HCC97H cells were transfected into HCC cell lines HCC97L-FL, HCC97H-FL with stable expression of luciferase. A total of 24 nude mice were randomly divided into HCC97L-FL group and HCC97H-FL group by the random number table method. Cell suspension was prepared by adding HCC97L-FL, HCC97H-FL cells (5×10⁶) into the phosphate buffered saline (PBS, 50 μl) respectively. Cell suspension was injected into the caudal vein of nude mice. The model of tracking pulmonary metastasis of HCC with fluorescein was established. The gross and tissue samples of the pulmonary metastasis tracked by fluorescence imaging were observed. The data between two groups were compared using t test.

Through fluorescence detection, intensive fluorescence could be observed in the HCC97L-FL, HCC97H-FL cells. After 2 to 3 weeks of modeling, fluorescence signal was collected from the nude mice lung in HCC97L-FL group. The fluorescence intensity enhanced gradually in 3-4 week. No fluorescence signal was observed in the nude mice lung in HCC97H-FL group. After 4 weeks of modeling, the fluorescence intensity was (1.73 ± 0.16) ×10⁴ a.u. in HCC97H-FL group, which was significantly higher than that in HCC97L-FL group [(0.03 ±0.01) ×10⁴ a.u.] (t=23.652, P<0.05). The nude mice lung in HCC97H-FL group was densely covered with metastatic tumors of varying sizes after 4 weeks of modeling. Some of the bigger tumors were visible on the lung surface. No metastatic tumor was observed in the HCC97L-FL group. The metastatic tumor was observed with an obvious cell nuclear atypia and characteristic feature of HCC.

Fluorescence imaging of using luciferase to label HCC cells can dynamically track the pulmonary metastasis of HCC of nude mouse . This technique provides a reliable quantitative research method for studying the growth, invasion and metastasis of HCC cells in vivo.