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中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2019, Vol. 08 ›› Issue (02) : 175 -178. doi: 10.3877/cma.j.issn.2095-3232.2019.02.020

基础研究

Id-1在肝细胞癌中表达及其在肿瘤转移中作用机制探讨
艾军华1, 刘刚1, 程素芬1, 项灯1, 熊振芳2, 时军1,†()   
  1. 1. 330006　南昌大学第一附属医院肝胆移植科
    2. 330006　南昌大学第一附属医院病理科
  • 收稿日期:2019-01-04 出版日期:2019-04-10
  • 通信作者: 时军
  • 基金资助:
    国家自然科学基金地区基金资助项目(81450045)

Expression of Id-1 and its mechanism in the metastasis of hepatocellular carcinoma

Junhua Ai1, Gang Liu1, Sufen Cheng1, Deng Xiang1, Zhenfang Xiong2, Jun Shi1,†()   

  1. 1. Department of Hepatobiliary Transplantation, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
    2. Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
  • Received:2019-01-04 Published:2019-04-10
  • Corresponding author: Jun Shi
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艾军华, 刘刚, 程素芬, 项灯, 熊振芳, 时军. Id-1在肝细胞癌中表达及其在肿瘤转移中作用机制探讨[J]. 中华肝脏外科手术学电子杂志, 2019, 08(02): 175-178.

Junhua Ai, Gang Liu, Sufen Cheng, Deng Xiang, Zhenfang Xiong, Jun Shi. Expression of Id-1 and its mechanism in the metastasis of hepatocellular carcinoma[J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2019, 08(02): 175-178.

目的

探讨分化抑制因子-1（Id-1）在肝细胞癌（肝癌）组织中的表达及其在肿瘤转移中的作用机制。

方法

标本来源于2014年1月至2016年12月在南昌大学第一附属医院接受肝切除的56例肝癌患者组织标本。患者均签署知情同意书，符合医学伦理学规定。其中男41例，女15例；年龄26~79岁，中位年龄55岁。45例癌旁肝组织和21例正常肝组织为对照。采用免疫组化法检测Id-1、E-cadherin、Vimentin和MMP-9表达。两组阳性率比较采用χ2检验，并进行Spearman等级相关分析。

结果

肝癌组织Id-1、E-cadherin、Vimentin、MMP-9表达阳性率分别为86%（48/56）、75%（42/56）、39%（22/56）、84%（47/56），明显高于对照组的62%（41/66）、33%（22/66）、15%（10/66）、47%（31/66）（χ2=8.57，13.62，10.73，11.08；P<0.05)。肿瘤直径>5 cm患者Id-1阳性率为100%（24/24），明显高于直径≤5 cm患者的75%（24/32）；转移患者Id-1阳性率97%（34/35），亦明显高于无转移患者的66%（14/21） （χ2=9.25，10.24；P<0.05）。Id-1表达强度与肝癌分化程度呈负相关（rs=-0.295，P<0.05）。Id-1表达与E-cadherin、MMP-9表达呈负相关（rs=-0.432，-0.326；P<0.05），而与Vimentin表达呈正相关（rs=0.713，P<0.05）。

结论

Id-1在肝癌中高表达，其可能机制为通过诱导上皮-间质转化和降解细胞外基质而促进肝癌侵袭转移。

关键词: 癌，肝细胞, 肿瘤侵润, 肿瘤转移, 上皮细胞-间充质细胞转换
Objective

To investigate the expression of inhibitor of differentiation-1 (Id-1) in hepatocellular carcinoma (HCC) and its mechanism in tumor metastasis.

Methods

Tissue specimens were collected from 56 HCC patients undergoing hepatectomy in the First Affiliated Hospital of Nanchang University from January 2014 to December 2016. The informed consents of all patients were obtained and the local ethical committee approval was received. Among them, 41 patients were male and 15 female, aged 26-79 years with a median age of 55 years. The para-carcinoma liver tissues from 45 cases and normal liver tissues from 21 healthy subjects were used as controls. The expression levels of Id-1, E-cadherin, Vimentin and MMP-9 were quantitatively measured by immunohistochemistry. The positive rates between two groups were compared by Chi-square test. Spearman's rank correlation analysis was performed.

Results

In HCC tissues, the positive rates of Id-1, E-cadherin, Vimentin and MMP-9 expression were 86%(48/56), 75%(42/56), 39%(22/56) and 84%(47/56), significantly higher compared with 62%(41/66), 33%(22/66), 15%(10/66) and 47%(31/66) in control group (χ2=8.57, 13.62, 10.73, 11.08; P<0.05). The positive rate of Id-1 in patients with tumor diameter >5 cm was 100%(24/24), significantly higher than 75%(24/32) in patients with tumor diameter≤5 cm. The positive rate of Id-1 in patients with tumor metastasis was 97%(34/35), significantly higher than 66%(14/21) in patients without tumor metastasis (χ2=9.25, 10.24; P<0.05). The expression level of Id-1 was negatively correlated with the degree of HCC differentiation (rs=-0.295, P<0.05). The expression level of Id-1 was negatively associated with the expression of E-cadherin and MMP-9 (rs=-0.432, -0.326; P<0.05), whereas positively correlated with the expression of Vimentin (rs=0.713, P<0.05).

Conclusions

Id-1 is highly expressed in HCC tissues probably by inducing epithelial-mesenchymal transition and degrading extracellular matrix, thereby promoting the invasion and metastasis of HCC.

Key words: Carcinoma, hepatocellular, Neoplasm invasiveness, Neoplasm metastasis, Epithelial-mesenchymal transition
图1 肝癌和癌旁组织Id-1表达（免疫组化法×100） 注：a为肝癌组织，b为癌旁组织；Id-1为分化抑制因子-1
表1 Id-1表达表达与肝癌组织分化程度相关性分析（例）
分化程度 Id-1
- + ++
低分化 0 3 10
中分化 1 10 14
高分化 7 10 1
表2 Id-1、E-cadherin、Vimentin和MMP-9表达的相关性（例）
Id-1 E-cadherin Vimentin MMP-9
- + ++ - + ++ - + ++
- 6 1 1 6 3 0 0 3 6
+ 7 13 3 11 7 4 3 7 13
++ 1 6 18 17 4 4 6 13 5
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