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中华肝脏外科手术学电子杂志

中华肝脏外科手术学电子杂志 ›› 2018, Vol. 07 ›› Issue (02) : 147 -151. doi: 10.3877/cma.j.issn.2095-3232.2018.02.015

所属专题： 文献；

基础研究

端粒酶调控因子PinX1抑制肝癌细胞增殖与侵袭
李瑞曦1, 姚志成2, 熊志勇2, 周伯宣1, 徐见亮1, 胡昆鹏2, 范伟明1, 梁豪1, 邓美海1,†()   
  1. 1. 510630　广州，中山大学附属第三医院肝胆外科
    2. 510530　广州，中山大学附属第三医院岭南医院普通外科
  • 收稿日期:2018-01-15 出版日期:2018-04-10
  • 通信作者: 邓美海
  • 基金资助:
    广东省自然科学基金(2016A030313848，S2013010016015); 广州市科技计划项目(2013J4100061)

Telomerase regulation factor PinX1 inhibits proliferation and invasion of hepatoma cells

Ruixi Li1, Zhicheng Yao2, Zhiyong Xiong2, Boxuan Zhou1, Jianliang Xu1, Kunpeng Hu2, Weiming Fan1, Hao Liang1, Meihai Deng1,†()   

  1. 1. Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    2. Department of General Surgery, Lingnan Hospital, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510530, China
  • Received:2018-01-15 Published:2018-04-10
  • Corresponding author: Meihai Deng
  • About author:
    Corresponding author: Deng Meihai, Email:
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李瑞曦, 姚志成, 熊志勇, 周伯宣, 徐见亮, 胡昆鹏, 范伟明, 梁豪, 邓美海. 端粒酶调控因子PinX1抑制肝癌细胞增殖与侵袭[J/OL]. 中华肝脏外科手术学电子杂志, 2018, 07(02): 147-151.

Ruixi Li, Zhicheng Yao, Zhiyong Xiong, Boxuan Zhou, Jianliang Xu, Kunpeng Hu, Weiming Fan, Hao Liang, Meihai Deng. Telomerase regulation factor PinX1 inhibits proliferation and invasion of hepatoma cells[J/OL]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2018, 07(02): 147-151.

目的

探讨端粒酶调控因子PinX1对肝癌细胞增殖和侵袭能力的影响。

方法

构建稳定表达PinX1的肝癌细胞PinX1-7721（实验组）及其对照细胞VECTOR-7721（对照组）。RT-PCR法检测PinX1 mRNA的表达，CCK-8法和平板克隆形成实验检测肝癌细胞的增殖能力和克隆形成能力，Transwell实验检测肝癌细胞的侵袭能力。实验数据比较采用t检验。

结果

实验组细胞PinX1 mRNA表达量为（13.9±2.0）×10-3，明显高于对照组的（1.1±0.2）×10-3（t=10.98，P<0.05）。实验组细胞1～7 d的A450值分别为0.260±0.004、0.340±0.008、0.450±0.040、0.500±0.020、0.730±0.030、1.350±0.040、1.640±0.050，明显低于对照组的0.280±0.009、0.410±0.007、0.680±0.044、0.730±0.029、0.850±0.070、1.700±0.020、2.080±0.280（t=-5.82，-12.99，-6.36，-5.96，-28.42，-18.98，-5.08；P<0.05）。平板克隆实验结果显示实验组细胞克隆形成数目为（143±32）个，明显低于对照组的（305±25）个（t=-6.91，P<0.05）。Transwell实验结果显示实验组细胞穿膜数目为（230±16）个，明显低于对照组的（650±30）个（t=-21.40，P<0.05）。

结论

PinX1可抑制肝癌细胞的增殖和侵袭能力。

关键词: 癌，肝细胞, 端粒酶, PinX1, hTERT
Objective

To explore the impact of telomerase regulation factor PinX1 to the proliferation and invasion ability of hepatoma cells.

Methods

Hepatoma cells PinX1-7721 (experimental group) with stable expression of PinX1 as well as control cell VECTOR-7721 (control group) were constructed. The expression of PinX1 mRNA was detected by RT-PCR. The proliferation ability and clonality of hepatoma cells were detected by CCK-8 method and plate clonality assay, and the invasion ability of hepatoma cells by Transwell assay. Comparison of the experiment data was conducted by t test.

Results

Expression level of PinX1 mRNA in experiment group was (13.9±2.0)×10-3, which was significantly higher than (1.1±0.2)×10-3 in control group (t=10.98, P<0.05). A450 of the cells on 1-7 d in experiment group was respectively 0.260±0.004, 0.340±0.008, 0.450±0.040, 0.500±0.020, 0.730±0.030, 1.350±0.040 and 1.640±0.050, which were significantly lower than 0.280±0.009, 0.410±0.007, 0.680±0.044, 0.730±0.029, 0.850±0.070, 1.700±0.020 and 2.080±0.280 in control group (t=-5.82, -12.99, -6.36, -5.96, -28.42, -18.98, -5.08; P<0.05). The plate clonality assay results showed that the clone formation quantity of cells in experiment group was 143±32, which was significantly lower than 305±25 in control group (t=-6.91, P<0.05). Transwell assay results showed that the quantity of trans-membrane cell in experiment group was 230±16, which was significantly lower than 650±30 in control group (t=-21.40, P<0.05).

Conclusion

PinX1 could inhibit the proliferation and invasion ability of hepatoma cells.

Key words: Carcinoma, hepatocellular, Telomerase, PinX1, hTERT
图1 实验组与对照组肝癌细胞生长曲线
图2 实验组与对照组肝癌细胞平板克隆形成实验
图3 实验组与对照组肝癌细胞Transwell实验结果（×100）
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